NDSB-195 [160255-06-1]

NDSB-195 is protein stabilizer that enhances the dynamics of the β4-α2 loop of ubiquitin. NDSB-195 stabilizes Saccharomyces cerevisiae ubiquitin against guanidium chloride denaturation, increasing the onset temperature of heat denaturation in the presence of the denaturant^[1].

NDSB-195 (0.5-1.0 M) stabilizes S. cerevisiae ubiquitin against guanidium chloride-induced denaturation, as shown by preserved secondary structure and an increased heat denaturation onset temperature of 70°C with 0.5 M NDSB-195^[1].
NDSB-195 (0-500 mM) selectively reduces the amide proton NMR line width of Gly53 in yeast ubiquitin in a concentration-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Wang H, et al. Protein stabilizer, NDSB-195, enhances the dynamics of the β4 -α2 loop of ubiquitin. J Pept Sci. 2016 Mar;22(3):174-80.