NADP-Malate Dehydrogenase (NADP-MDH) Activity Assay Kit
Cat# E-BC-K562-M-96T
Size : 96T
Brand : Elabscience
Contact local distributor :
Phone : +1 850 650 7790
NADP-Malate Dehydrogenase (NADP-MDH) Activity Assay Kit (E-BC-K562-M)
For research use only.
| Detection Principle | NADP-MDH catalyzes the conversion of malic acid and NADP+ to oxaloacetic acid and NADPH. NADPH makes WST-8 orange under the action of electron coupling agent. The activity of NADP-MDH can be calculated by measuring the change of absorbance value at 450 nm. |
| Synonyms | NADP-MDH |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (440-460 nm) |
| Research Area | Mitochondria Function , Tricarboxylic Acid（TCA）Cycle , Energy Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.63 U/L |
| Detection Range | 0.63-50.0 U/L |
| Precision | inter-assay CV: 6.4% | intra-assay CV: 1% |
| Sample Volume | 20 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat kidney tissue homogenate | 3-10 |
| 10% Rat heart tissue homogenate | 1-5 |
| 10% Mouse liver tissue homogenate | 1-10 |
| 10% Mouse lung tissue homogenate | 3-10 |
| 10% Mouse kidney tissue homogenate | 3-10 |
| 10% Epipremnum aureum tissue homogenate | 1 |
| Mouse serum | 1 |
| Rat serum | 1 |
| Human serum | 1 |
| Rat plasma | 1 |
| HT29 cell | 1 |
| Molt-4 cell | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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