Mouse Kappa Chain Antibody - HRP Conjugated
Cat# OARA05032
Size : 1mg
Brand : Aviva Systems Biology
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Rabbit Anti-Mouse ÃŽÃâ32)
| Datasheets/Manuals | Printable datasheet for Rabbit Anti-Mouse ÃŽÃâ32) |
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| Predicted Species Reactivity | Mouse |
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| Product Format | Lyophilized. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | HRP |
| Application | ELISA|IHC|WB |
| Reconstitution and Storage | -20°C or -80°C|2°C to 8°C |
| Immunogen | Anti-Mouse kappa light chain was produced by repeated immunization with Mouse kappa light chain in rabbit. |
| Purification | This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. |
| Concentration | 1 mg/ml |
| Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes. |
| Application Info | ELISA: 1:10000~50000 IHC: 1:500~2500 WB: 1:2000~5000 Anti-Mouse kappa light chain antibody generated in rabbit detects specifically Mouse kappa light chain. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. |
| Alias Symbols | HRP |
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| Description of Target | Anti-Mouse κ (kappa chain) (RABBIT) Antibody generated in rabbit detects specifically Mouse kappa light chain. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). The light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG kappa is conjugated to peroxidase. |
| Molecular Weight | 40 kDa |