Product Name

Mouse CRP / C Reactive Protein / PTX1 ELISA Kit PicoKine®

SKU/Catalog Number

EK0977

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Acute-phase response (putative; GO:0006953) with hepatic CRP induction downstream of innate immune activation (putative; TLR→IL-6 axis); clinical utility (putative): inflammation/sepsis monitoring. Assay context: sandwich ELISA for mouse serum/plasma (heparin/EDTA), lysates and culture supernatants; sensitivity ~10 pg/mL; range 156–10,000 pg/mL (from product page). Often profiled alongside TNF-α, IL-6, and LBP in inflammatory panels (putative).

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse CRP / C Reactive Protein / PTX1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0977)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: H20-S225

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

See datasheet of EK0977 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0977 is reactive to Crp in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0977 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Crp

C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.

Kit Components

Catalog Number	Description	Quantity
EK0977-CAP	Anti-Mouse Crp Pre-coated 96-well strip microplate	1
EK0977-ST	Mouse Crp Standard	2 vials, 10 ng/tube
EK0977-DA	Mouse Crp Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.01	0.078	0.135	0.246	0.436	0.819	1.433	2.107

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Crp,

Application	Dilution	Species
Dilution ratio of 1:1000	concentration in serum and plasma is around 1 ug/ml.

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Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse CRP / C Reactive Protein / PTX1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.