Reagents and instruments for immunology, cell biology and molecular biology.
 
> mouse Anti-Tyrosine, phosphorylated (Tyr) Monoclonal Antibody [Clone:3A41(P-Tyr-100)]

mouse Anti-Tyrosine, phosphorylated (Tyr) Monoclonal Antibody [Clone:3A41(P-Tyr-100)]

Print Print
Please log in

Cat# T9228-75B-100ul

Size : 100ul

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790

T9228-75B Tyrosine, phosphorylated (Tyr)

Clone Type
Monoclonal
Host
mouse
Isotype
IgG1
Grade
Ascites
Applications
E FC IC IF IHC IP WB
Crossreactivity
Bo Ca Ch Gt Hu Mo Rb Rt Sh
Shipping Temp
Blue Ice
Storage Temp
-20°C

Tyrosine phosphorylation plays a key role in cellular signaling. Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals. Antibodies specific for phospho-tyrosine have been invaluable reagents in these studies. Phospho-tyrosine monoclonal antibodies are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry and Flow Cytometry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:2000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunoprecipitation: 1:100
Immunohistochemistry (Paraffin): 1:4800. Use EDTA as unmasking buffer.
ELISA (Peptide): 1:4000
Immunofluorescence (IC): 1:1600
Flow Cytometry: 1:3200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 3A41(P-Tyr-100)|Host: mouse|Concentration: Not Determined |Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.|Purity: Ascites|Immunogen: Phospho-tyrosine-containing peptides|Specificity: Recognizes phospho-Tyr in a manner largely independent of the surrounding amino acid sequence in ELISA. Binds to a larger number of phospho-Tyr-containing peptides than any other commercially available anti-phospho-Tyr monoclonal antibody. Does not crossreact with peptides containing phospho-Ser or phospho-Thr. Species Crossreactivity: All species||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Phospho-tyrosine-containing peptides
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity
Ascites
Specificity
Recognizes phospho-Tyr in a manner largely independent of the surrounding amino acid sequence in ELISA. Binds to a larger number of phospho-Tyr-containing peptides than any other commercially available anti-phospho-Tyr monoclonal antibody. Does not crossreact with peptides containing phospho-Ser or phospho-Thr. Species Crossreactivity: All species
References
1. Asao, H. et al. (2001). J.Immunol. 167: 1-5. 2. Chang, C.I. et al. (2000) . J.Immunol. 165: 2134-2141. 3. Farhang-Fallah, J. et al. (2000) J. Biol. Chem. 275: 40492-40497. 4. Kaluz, S. et al. (2002) Cancer Res. 62: 4469-4477. 5. Kato, R. et al. (2002). Clin. Cancer Res. 8: 2455-2462. 6. Klein, R.D. and Fischer, S.M. (2002) Carcinogenesis 23(1): 217-221. 7. Perez, O.D. and Nolan, G.P. (2002) Simultaneous measurement of multiple active kinase states using polychromatic flow cytometry. Nat. Biotechnol. 20(2): 155-162. 8. Stewart, D.M. et al. (2001) Endocrinology 142(1): 98-107.11. Varmus, H. and Bishop, J.M. (1986) Cancer Surv. 5: 153-158. 12. Hunter, T. and Cooper, J.A. (1985) Annu. Rev. Biochem. 54: 897-930. 13. Fischer, E.H. (1999) Adv. Enzyme Regul. 39: 359-69. 14. Hunter, T. (1997) Philos. Trans. R. Soc. Lond. B. Biol. Sci. 353: 583-605. 15. Ward, S.G. et al. (1992) J. Biol. Chem. 267: 23862-9. 16. Glenney, J.R. (1988) J. Immunol. Methods 109: 277-285.