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> mouse Anti-HLA Class I (Low Endotoxin, Azide Free) Monoclonal Antibody [Clone:W6/32]

mouse Anti-HLA Class I (Low Endotoxin, Azide Free) Monoclonal Antibody [Clone:W6/32]

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Cat# 366956-100ug

Size : 100ug

Brand : US Biological

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Phone : +1 850 650 7790

366956 HLA Class I (Low Endotoxin, Azide Free)

Clone Type
Monoclonal
Host
mouse
Source
human
Swiss Prot
P30443, Q96DW9, O19617, P13747
Isotype
IgG2a
Grade
Affinity Purified
Applications
E FC IC IHC IP
Crossreactivity
Bo Fe Hu Mk
Shipping Temp
Blue Ice
Storage Temp
-20°C

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumor defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

366956 is a valuable reagent for analyzing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. Also suitable as a positive control for HLA tissue typing and crossmatching.

Applications:
Suitable for use in ELISA, Flow Cytometry, Immunoprecipitation, Immunohistochemistry and Functional Application. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1-4ug/ml
Immunohistochemistry: frozen
Functional Application: suitable as a positive control for HLA tissue typing.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG2a|Clone No: W6/32|Host: mouse|Source: human|Concentration: ~1mg/ml|Form: Supplied as a liquid in PBS, pH 7.4. No preservative added.|Purity: Purified by Protein A affinity chromatography.|Immunogen: Membrane of human tonsil cells|Specificity: Recognizes an extracellular epitope of human MHC Class I molecules (MHC Class Ia). Species Crossreactivity: bovine, primate and feline. Does not react with rabbit.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Membrane of human tonsil cells
Form
Supplied as a liquid in PBS, pH 7.4. No preservative added.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes an extracellular epitope of human MHC Class I molecules (MHC Class Ia). Species Crossreactivity: bovine, primate and feline. Does not react with rabbit.
References
1. Barnstable, C.J., et al. (1978). Cell. 14(1):9-20. |2. Brodsky, F.M. et al. (1982): Evolution of HLA antigenic determinants: species cross reactions of monoclonal antibodies. Immunogenetics 15: 151-166.|3. Stern, P. et al. (1987): Class I-like MHC molecules expressed by baboon placental synctiotrophoblast. Journal of Immunology. 138 (4): 1088 - 1091.|4. Jacobsen, C. N. et al. (1993): Reactivities of 20 anti-human monclonal antibodies with leucocytes from ten different animal species. Vet. Immunopathol. 39: 461 - 466.|5. Shields MJ, Ribaudo RK: Mapping of the monoclonal antibody W6/32: sensitivity to the amino terminus of beta2-microglobulin. Tissue Antigens 1998 May; 51(5):567-70.