mouse Anti-CD8 (Leu2 T lymphocyte antigen, MAL, OKT8 T Cell antigen, p32, T cell antigen Leu2, T cell surface glycoprotein CD8 alpha, Suppressor/Cytotoxic T cell) (Biotin) Monoclonal Antibody [Clone:5K112 (fCD8)]

Feline CD8, a member of the immunoglobulin superfamily of cell surface receptors, is a type II transmembrane glycoprotein that is expressed as a heterodimer on the “suppressor/cytotoxic” subpopulation of peripheral T lymphocytes. It is present on approximately 63% of thymocytes, 9% of splenocytes, 20% of lymph node cells, and 15% of peripheral blood lymphocytes.1,2 CD8 functions as a co-receptor with MHC Class I-restricted T cell receptors in antigen recognition.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: Frozen sections (acetone fixed).
Flow Cytometry: ≤ 1ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.