MMP9 High Sensitivity ELISA Kit (Horse)
Cat# OKCD01327
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
MMP9 High Sensitivity ELISA Kit (Horse) (OKCD01327)
| Datasheets/Manuals | Printable datasheet for MMP9 High Sensitivity ELISA Kit (Horse) (OKCD01327) |
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| Predicted Species Reactivity | Equus caballus|Horse | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
| ELISA Kit Duration | 3h | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Matrix Metalloproteinase 9 (MMP9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Matrix Metalloproteinase 9 (MMP9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Matrix Metalloproteinase 9 (MMP9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Matrix Metalloproteinase 9 (MMP9) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
| ELISA Kit Range | 15.6-1000pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Matrix Metalloproteinase 9 (MMP9) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Matrix Metalloproteinase 9 (MMP9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | -20°C|2°C to 8°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 5.9 pg/mL | ||||||||||||||||||||||
| Specificity | This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 9 (MMP9). No significant cross-reactivity or interference between Matrix Metalloproteinase 9 (MMP9) and analogues was observed. | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | MMP9 |
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| Gene Full Name | matrix metallopeptidase 9 |
| Alias Symbols | 92 kDa gelatinase, gelatinase B, matrix metalloproteinase 9, matrix metalloproteinase-9, type IV collagenase. |
| NCBI Gene Id | 100056599 |
| Protein Name | MMP9 |
| Uniprot ID | A8HDR2 |
| Protein Accession # | NP_001104772.1 |
| Nucleotide Accession # | NM_001111302.1 |
