Immunoprecipitation. Functional Assays. Flow Cytometry: Use 10µl of the suggested working dilution to label 1x10e6 cells in 100µl. Immunohistochemistry on frozen sections: 1/100.
Reactivity
Human
Antibody Host
Mouse
Isotype
IgG2a
Clonality
Monoclonal
Immunogen
MICA transfected C1R cells. Spleen cells from immunised RBF/DnJ mice were fused with cells of the P3 mouse myeloma cell line.
Specificity
This antibody is specific for nonclassical MHC class I chain A (MICA) and nonclassical MHC class I chain MICB (MICB).
MICA and MICB are stress inducible antigens, which are closely related and appear functionally indistinguishable. MICA and MICB are ligands for NKG2D, an activating receptor on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. MICA is principally expressed on intestinal epithelium, and several epithelial tumours. Expression may be induced to high surface levels by heat shock, oxidative stress, and virus infection. Clone 6D4 is reported to inhibit the cytotoxicity of NK cells stimulated by IFN alpha-treated dendritic cells.
Synonyms
MHC class I polypeptide-related sequence A, MIC-A, PERB11.1
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