Specifications

Product Data
Locus ID	9833
Synonyms	HPK38
Vector	pGFP-C-shLenti
E. coli Selection	Chloramphenicol (34 ug/ml)
Mammalian Cell Selection	Puromycin
Format	Lentiviral plasmids
Components	MELK - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 9833). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_001256685, NM_001256687, NM_001256688, NM_001256689, NM_001256690, NM_001256691, NM_001256692, NM_001256693, NM_014791, NR_046337, NM_014791.1, NM_014791.2, NM_014791.3, NM_001256693.1, NM_001256692.1, NM_001256691.1, NM_001256688.1, NM_001256690.1, NM_001256687.1, NM_001256685.1, NM_001256689.1, BC014039, BC014039.2
UniProt ID	Q14680
Summary	Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.UniProtKB/Swiss-Prot Function
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact tech@quimigen.pt. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage	Store at -20°C
Shipping	Ambient

Product Manuals

HuSH-29 shRNA Plasmids HuSH pRS Cloning Vector HuSH RNAi Validation Vector HuSH pGFP-V-RS Cloning Vector

FAQs

shRNA (HuSH-29)

SDS

SDS for TL320621

RNAi Resources

Product overview Video shRNA Selection Guide FAQ: shRNA FAQ: siRNA FAQ; miRNA Product Brochure: RNAi QC Data for Lenti shRNA