MAGIC RED CATHEPSIN B KIT
Cat# OKSA11290
Size : 25Tests
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
MAGIC RED CATHEPSIN B KIT (OKSA11290)
| Datasheets/Manuals | Printable datasheet for MAGIC RED CATHEPSIN B KIT (OKSA11290) |
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| Predicted Species Reactivity | Human |
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| Product Format | Kit |
| Application | IF |
| ELISA Kit Component | Magic RedTM MR-(RR)2 Hoechst 33342 Stain Acridine orange (AO) |
| Reconstitution and Storage | 2°C to 8°C |
| Specificity | CATHEPSIN B |
| Description of Target | Cathepsin Magic RedTM kits measure cathepsin B protease activity by detecting active cathepsins in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of cathepsins to produce a fluorescent product. The Magic RedTM reagent contains a cathepsin B target sequence peptide (RR)2 linked to a red (Cresyl Violet) fluorescent probe. The Cathepsin Magic RedTM kits are suitable for cells in suspension, adherent cells, thin frozen tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits. This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates. |
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