LKB1 (STK11) (NM_000455) Human Tagged ORF Clone
Cat# RC202885
Size : 10ug
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LKB1 (STK11) (NM_000455) Human Tagged ORF Clone
SKU
RC202885
STK11 (Myc-DDK-tagged)-Human serine/threonine kinase 11 (STK11)
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | LKB1 |
| Synonyms | hLKB1; LKB1; PJS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms | Chromatograms  Sequencher program is needed, download here |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene | Plasmid Map  |
| ACCN | NM_000455 |
| ORF Size | 1299 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000455.5 |
| RefSeq Size | 3286 bp |
| RefSeq ORF | 1302 bp |
| Locus ID | 6794 |
| UniProt ID | Q15831, A0A0S2Z4D1 |
| Cytogenetics | 19p13.3 |
| Domains | pkinase, S_TKc, TyrKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Adipocytokine signaling pathway, mTOR signaling pathway |
| MW | 48.5 kDa |
| Summary | This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. provided by RefSeq, Jul 2008 |
| Product Manuals |
| FAQs |
|
| SDS |
| SKU | Description | Size | ||
|---|---|---|---|---|
| RC202885L1 | Lenti ORF clone of Human serine/threonine kinase 11 (STK11), Myc-DDK-tagged | 10 ug | | |
| RC202885L2 | Lenti ORF clone of Human serine/threonine kinase 11 (STK11), mGFP tagged | 10 ug | | |
| RC202885L3 | Lenti ORF clone of Human serine/threonine kinase 11 (STK11), Myc-DDK-tagged | 10 ug | | |
| RC202885L4 | Lenti ORF clone of Human serine/threonine kinase 11 (STK11), mGFP tagged | 10 ug | | |
| RG202885 | STK11 (tGFP-tagged) - Human serine/threonine kinase 11 (STK11) | 10 ug | | |
| SC119871 | STK11 (untagged)-Human serine/threonine kinase 11 (STK11) | 10 ug | | |
| SC323432 | STK11 (untagged)-Kinase deficient mutant (K78M) of Human serine/threonine kinase 11 (STK11) | 10 ug | | |
Citations
HECTD3 enhances cell radiation resistance and migration by regulating LKB1 mediated ZEB1 in glioma
Yuan, Liu, Fan et al
Eur J Neurosci (2022)
Yuan, Liu, Fan et al
Eur J Neurosci (2022)
Cited by 4
AI Summary: OriGene products—including the HECTD3 lentiviral vector (RC220357L3V), LKB1 expression plasmid (RC202885), shRNA-HECTD3 (TL304132V), shRNA-LKB1 (TL301341V), and scramble controls—were used to manipulate gene expression in glioma cells, enabling the study of how HECTD3 regulates radiation resistance and migration via LKB1 and ZEB1 pathways.
Snippet: The lentiviral vectors of HECTD3 (cat no. RC220357L3V), expression plasmids of LKB1 (cat no. RC202885), shRNA-HECTD3 (shHECTD3;cat no. TL304132V) and the shRNA of LKB1 (shLKB1, cat no. TL301341V) were purchased from Origene (Beijing, China), as well as the scramble control (NC and shNC). The lentiviral vectors used to upregulate ZEB1
Genome-wide RNAi screen reveals disease-associated genes that are common to Hedgehog and Wnt signaling
Jacob, Wu, Dodge et al
Sci Signal (2011) 4 (157), ra4 DOI: 10.1126/scisignal.2001225
Jacob, Wu, Dodge et al
Sci Signal (2011) 4 (157), ra4 DOI: 10.1126/scisignal.2001225
Cited by 96
AI Summary: OriGene contributed to this study by providing the human STK11 (NM_000455) expression construct, which was used to investigate the role of STK11 in Hedgehog and Wnt signaling pathways.
Snippet: revealed that the integration of RNAi-derived data sets is limited by the effectiveness of the siRNA design and current high-throughput screening formats and showed the need to screen ... The human STK11 (NM_000455) expression construct was purchased from Origene.
Showing 1-2 of 2 papers.
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