Malondialdehyde (MDA) is one of the products of lipid peroxidation in organisms and is widely used as a detection indicator for lipid peroxidation. A lipid peroxidation (MDA) assay is a kit used to detect MDA in samples such as tissue, cell lysate, plasma, serum, and urine by thiobarbituric acid (TBA). The detection principle is that MDA reacts with TBA to produce a red product, which is specifically absorbed at 535 nm and can therefore be determined by colorimetry. At the same time, the reaction product can also be excited at 535 nm, producing a maximum emission wavelength of 553 nm, so fluorescence detection is also possible. The kit provides antioxidants that inhibit the production of new MDA in the sample during the detection, making the detection more accurate. At the same time, the kit can detect MDA as low as 1 μM, with a good linear relationship in the range of 1-200 μM.

Figure 1: Typical MDA assay standard curve