interferon alpha 5/IFNA5 Protein, Rat, Recombinant (His)

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Cat# NB-64-58218-100ug

Size : 100ug

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interferon alpha 5/IFNA5 Protein, Rat, Recombinant (His)

(Synonyms: interferon-α, interferon, α5, interferon, alpha 5, IFNA5) Copy Product Info

Synonyms: interferon-α, interferon, α5, interferon, alpha 5, IFNA5

Catalog No. TMPY-03130 Copy Product Info

interferon alpha 5/IFNA5 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 20.9 kDa and the accession number is A0A7R8C3L7.

For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity

Bioactivity	Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is typically 10-50 pg/mL.
Description	interferon alpha 5/IFNA5 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 20.9 kDa and the accession number is A0A7R8C3L7.
Species	Rat
Expression System	HEK293 Cells
Tag	C-His
Accession Number	A0A7R8C3L7
Construction	A DNA sequence encoding the rat IFNA5 (XP_001076062.1) (Met1-Glu189) was expressed, fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Cys 24
Protein Purity	> 95 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	interferon-α, interferon, α5, interferon, alpha 5, IFNA5
Research Background	Interferon, alpha 5 (IFNA5) belongs to the alpha/beta interferon family. IFNA5 is the only IFNA subtype detected in normal liver, while a mixture of subtypes is observed in the liver tissue of patients with chronic hepatitis C. Interferons are produced by macrophages, IFN-alpha has antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha, the first cytokine to be produced by recombinant DNA technology, has emerged as an important regulator of growth and differentiation, affecting cellular communication and signal transduction pathways as well as immunological control. Originally discovered as an antiviral substance, the efficacy of IFN-alpha in malignant, viral, immunological, angiogenic, inflammatory, and fibrotic diseases suggests a spectrum of interrelated pathophysiologies. IFN-alpha emerged as a prototypic tumor suppressor protein that represses the clinical tumorigenic phenotype in some malignancies capable of differentiation.

Chemical Properties

Molecular Weight	20.9 kDa (predicted); 21 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

interferon, α 5interferon, a 5IFNA-5IFNA 5

Related Tags: interferon alpha 5/IFNA5 Protein, Rat, Recombinant (His) chemical structure | interferon alpha 5/IFNA5 Protein, Rat, Recombinant (His) molecular weight