IL-17A ELISpot Assay Kit (Human)
Cat# OKDB00027-1x96Wells
Size : 1x96Wells
Brand : Aviva Systems Biology
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Phone : +1 850 650 7790
IL-17A ELISpot Assay Kit (Human) (OKDB00027)
| Datasheets/Manuals | Printable datasheet for IL-17A ELISpot Assay Kit (Human) (OKDB00027) |
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| Predicted Species Reactivity | Human |
|---|---|
| Application | ELISPOT |
| ELISA Kit Duration | < 4 Hours |
| ELISA Kit Principle | Aviva's Human IL-17A ELISpot Kit is based on standard sandwich ELISA methodology. A capture antibody highly specific for the analyte of interest is coated to the wells of a PVDF bottomed 96 well microtitre plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and washed. Cell suspension and stimulant are added and the plate incubated allowing the specific antibodies to bind any analytes produced. Cells are then removed by washing prior to the addition of Biotinylated detection antibodies which bind to the previously captured analyte. Enzyme conjugated streptavidin is then added binding to the detection antibodies. Following incubation and washing substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using a microscope |
| ELISA Kit Component | Reagents sufficient for 96 Wells: 1. Pre-coated 96 well PVDF bottomed plates (1x96 Wells) 2. Biotinylated detection antibody 3. Streptavidin-Alkaline Phosphatase conjugate 4. Bovine Serum Albumin 5. Ready to use BCIP/NBT substrate buffer |
| Reconstitution and Storage | 2 to 8C, Do not use past expiration date. |
| Sample Type | Cell Suspensions |
| Assay Info | Assay Methodology: Quantitative Sandwich ELISpot Immunoassay |
| Gene Symbol | IL-17A |
|---|---|
| Gene Full Name | interleukin 17A |
| Alias Symbols | IL17, CTLA8, IL-17, CTLA-8, IL-17A |
| NCBI Gene Id | 3605 |
| Protein Name | interleukin-17A |
| Description of Target | The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. |
| Uniprot ID | Q16552 |
| Protein Accession # | NP_002181.1 |
| Nucleotide Accession # | NM_002190.2 |
| Protein Size (# AA) | 155 |
| Molecular Weight | 18 kDa |
