1. Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 0.5-5 ng/mL. 2. Labeled biotin to IL-15 Protein, Human, Recombinant by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of IL-15 Protein, Human, Recombinant, Biotinylated bound to Anti-IL15 Antibody was 0.1nM. (Routinely tested)

IL-15 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 12.8 kDa and the accession number is P40933-1.

Human

E. coli

≥ 95 % as determined by SDS-PAGE.

The protein encoded by the IL15 gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietic receptor subunits and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively, spliced transcript variants of this gene have been reported.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy