This multiplex phospho-array detects 55 phosphorylated human proteins from 5 signaling pathways: MAPK, AKT, JAK/STAT, NFκB, and TGFβ. Suitable for all liquid sample types, but intended for use with cell and tissue lysates.
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RayBiotech’s Human Phosphorylation Pathway Profiling Array identified oncogenic pathway alterations in glioblastoma cells overexpressing sst5TMD4. Figure adapted from Fuentes-Fayos et al., Int. J. Mol. Sci., 2022.
Human Phosphorylation Pathway Profiling Array C55
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 55 |
| Recommended Applications | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Research Area | Post-Translational Modifications, Phosphorylation, JAK/STAT Signaling, MAPK Signaling, PI3K-AKT Signaling, TGF-beta Signaling, NFkB Signaling, Akt Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
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Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| 4E-BP1 (P-Thr36)
| AKT (P-Ser473)
| ATF2 (P-Thr69/71)
| ATM (P-Ser1981)
| |
| BAD (P-Ser112)
| C-Fos (P-Thr232)
| CREB (P-Ser133)
| EGFR (P-Ser1070)
| |
| ERK1/2 (P-Thr202/P-Tyr204/P-Tyr185/P-Tyr187)
| GSK-3 alpha (P-Ser21)
| GSK-3 beta (P-Ser9)
| HDAC2 (P-Ser394)
| |
| HDAC4 (P-Ser632)
| HSP27 (P-Ser82)
| IKBa (P-Ser32)
| JAK1 (P-Tyr1022)
| JAK2 (P-Tyr1007/1008)
|
| JNK (P-Thr183)
| MEK (P-Ser217/221)
| MKK3 (P-Ser189)
| MKK6 (P-Ser207) (MEK6)
| MSK1 (P-Ser376)
|
| MSK2 (P-Ser360)
| mTOR (P-Ser2448)
| NF-kB (P-Ser536)
| p27 (P-Thr198)
| p38 (P-Thr180/Tyr182)
|
| P53 (P-Ser15)
| P70S6K (P-Thr421/Ser424)
| PDK1 (P-Ser241)
| PRAS40 (P-Thr246)
| PTEN (P-Ser380)
|
| Raf-1 (P-Ser301)
| RPS6 (P-Ser235/236)
| RSK1 (P-Ser380)
| RSK2 (P-Ser386)
| SHP-1 (P-Ser591)
|
| SHP2 (P-Tyr542)
| SMAD1 (P-Ser463/465)
| SMAD2 (P-Ser245/250/255)
| SMAD4 (P-Thr277)
| SMAD5 (P-Ser463/465)
|
| Src (P-Tyr419)
| STAT1 (P-Ser727)
| STAT2 (P-Tyr689)
| STAT3 (P-Tyr705)
| STAT5 (P-Tyr694)
|
| STAT6 (P-Tyr641)
| TAK1 (P-Ser412)
| TBK1 (P-Ser172)
| TYK2 (P-Tyr1054)
| ZAP70 (P-Tyr292)
|
Typical Data
Kit Components
- Human Phosphorylation Pathway Profiling Array C55 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 500X HRP-Anti-Rabbit IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentrate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray w/ Lid
- Protease Inhibitor Cocktail
- 100x Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
- Manual
Additional Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Assay Procedure Summary
- Block membranes
- Incubate with Sample
- Incubate with Detection Antibody Cocktail
- Incubate with HRP-Conjugated anti-IgG
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
Excel (AAH-PPP-1_AKT_Analysis_Tool.xls)
Excel (AAH-PPP-1_JAKSTAT_Analysis_Tool.xls)
Excel (AAH-PPP-1_MAPK_Analysis_Tool.xls)
Excel (AAH-PPP-1_NFkB_Analysis_Tool.xls)
Excel (AAH-PPP-1_TGFb_Analysis_Tool.xls)
citations
MCL1 modulates mTORC1 signaling to promote bioenergetics and tumorigenesis
- Species:
- Human
- Sample Type:
- Tissue Lysate / Homogenate (lysates from CHL-1 melanoma cells)
- Year:
- 2025
- Journal Name:
- Nature Communications
- PubMed ID:
- 41326406
The HRAS-binding C2 domain of PLCη2 suppresses tumor-like synoviocytes and experimental arthritis in rheumatoid arthritis
- Year:
- 2025
- Journal Name:
- Experimental and Molecular Medicine
- PubMed ID:
- 39894825
Integrative multi-omics identifies AP-1 transcription factor as a targetable mediator of acquired osimertinib resistance in non-small cell lung cancer
- Species:
- Human
- Sample Type:
- Cell Lysate (osimertinib-resistant HCC827 cells); Tissue Lysate / Homogenate
- Year:
- 2025
- Journal Name:
- Cell Death & Disease
- PubMed ID:
- 40414926
Persistent loss of membrane-associated MUC4 reprograms physiological functions in corneal epithelial cells via MAPK-dependent cascade
- Species:
- Human
- Sample Type:
- Tissue Lysate / Homogenate (HCE lysates)
- Year:
- 2026
- Journal Name:
- Scientific Reports
- PubMed ID:
- 42192195
Amelioration of premature aging in Werner syndrome stem cells by targeting SHIP/AKT pathway
- Year:
- 2025
- Journal Name:
- Cell Biosci
- PubMed ID:
- 39863890
Impaired splicing machinery in craniopharyngiomas unveils PRPF8 and RAVER1 as novel biomarkers and therapeutic targets
- Year:
- 2025
- Journal Name:
- Acta Neuropathologica Communications
- PubMed ID:
- 40581690
Assessing the diagnostic, prognostic, and therapeutic potential of the somatostatin/cortistatin system in glioblastoma
- Species:
- Human
- Sample Type:
- Other / Unspecified (primary patient-derived GBM cells)
- Year:
- 2025
- Journal Name:
- Cellular and Molecular Life Sciences
- PubMed ID:
- 40268793
Anticancer Activity of Novel Anilinoquinazoline Compounds against EGFR L858R/T790M-Mutant Non-Small Cell Lung Cancer in a Zebrafish Xenograft Model
- Species:
- Human
- Sample Type:
- Tissue Lysate / Homogenate (protein lysate)
- Year:
- 2026
- Journal Name:
- Research Square
Prostatic fibroblast reprogramming by Interleukin-30 activates prostate cancer metastasis programs
- Species:
- Human
- Sample Type:
- Cell Lysate (WPMY-1 cell lysates); Tissue Lysate / Homogenate
- Year:
- 2026
- Journal Name:
- Molecular biomedicine
- PubMed ID:
- 42340578
Identification of ligand-receptor pairs that drive human astrocyte development
- Year:
- 2023
- Journal Name:
- Nature Neuroscience
- PubMed ID:
- 37460808