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> Human MIF ELISA Kit

Human MIF ELISA Kit

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Cat# ELH-MIF-1

Size : 1PlateKit

Brand : RayBiotech

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Phone : +1 850 650 7790

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Human MIF ELISA KitHuman MIF ELISA KitHuman MIF ELISA KitHuman MIF ELISA KitHuman MIF ELISA KitHuman MIF ELISA Kit5 Plate Kit Packaging (one box)

Human MIF ELISA Kit

Human MIF ELISA Kit

RayBio® Human MIF ELISA Kit for cell culture supernatants, heparin treated plasma, and serum samples. EDTA and Citrate are not recommended.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Contact Us for Bulk Request

Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesHuman
Sensitivity
6 pg/ml

Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.

Detection Range
6 pg/ml - 6000 pg/ml
Specificity
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum
Recommended Dilution (Serum/Plasma)
2 fold
Accession Number
P14174
Gene Id
4282
Gene Symbols
MIF, GLIF, MMIF
Protein Name / Synonyms
Macrophage migration inhibitory factor (MIF) (EC 5.3.2.1) (Glycosylation-inhibiting factor) (GIF) (L-dopachrome isomerase) (L-dopachrome tautomerase) (EC 5.3.3.12) (Phenylpyruvate tautomerase)
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Research AreaCancer, Inflammation
Shipping TypeBlue ice
Storage≤-20°C

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Customize This Human MIF ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

  • Post-translational modifications (PTMs): Detect modified MIF, including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
  • Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
  • Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

documents

Datasheet (ELH-MIF.pdf)
SDS (ELH-MIF.pdf)
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)

citations

  • Monocytes and neutrophils promote cardiac fibroblast pro-fibrotic phenotypes through IL-6 and MIF

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (conditioned media from HCF with or without immune cell co-cultures)
    Year:
    2026
    Journal Name:
    Frontiers in Cell and Developmental Biology
    PubMed ID:
    42205676

  • Disruption of macrophage migration inhibitory factor signaling induces major tumor-associated macrophage phenotypes in human M2 macrophages

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (cell culture supernatants M2 macrophages)
    Year:
    2026
    Journal Name:
    Molecular biomedicine
    PubMed ID:
    42467313

  • MIF inhibition alleviates vitiligo progression by suppressing CD8+ T cell activation and proliferation

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2023
    Journal Name:
    Journal of Pathology

  • Efficacy of Sertraline Plus Placebo or Add-On Celecoxib in Major Depressive Disorder: Macrophage Migration Inhibitory Factor as a Promising Biomarker for Remission After Sertraline-Results From a Randomized Controlled Clinical Trial

    Species:
    Human
    Sample Type:
    Serum (MDD patients, baseline and week 6)
    Year:
    2021
    Journal Name:
    Frontiers in Psychiatry
    PubMed ID:
    34646168

  • The relationship of macrophage migration inhibitory factor with clinical and anamnestic parameters in patients with ST-segment elevation myocardial infarction

    Species:
    Human
    Year:
    2019
    Journal Name:
    Ukrainian Therapeutical Journal

  • Plasma Macrophage Migration Inhibitory Factor and CCL3 as Potential Biomarkers for Distinguishing Patients with Nasopharyngeal Carcinoma from High-Risk Individuals Who Have Positive Epstein-Barr Virus Capsid Antigen-Specific IgA

    Species:
    Human
    Sample Type:
    Plasma (Nasopharyngeal carcinom patients biomarker)
    Year:
    2019
    Journal Name:
    Cancer research and treatment
    PubMed ID:
    29807404

  • A pathwaybased approach to find novel markers of local glucocorticoid treatment in intermittent allergic rhinitis.

    Species:
    Human
    Sample Type:
    Nasal Wash
    Year:
    2011
    PubMed ID:
    20659080

  • The immunological role of monocytes and their cytokines in patients with breast cancer undergoing paravertebral analgesia

    Species:
    Human
    Sample Type:
    Serum (Analgesia breast cancer patients)
    Year:
    2015
    Journal Name:
    Comparative Clinical Pathology

  • Macrophage migration inhibitory factor (MIF) promoter polymorphisms (-794 CATT5-8 and -173 G>C): association with MIF and TNF in psoriatic arthritis

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2014

  • Vernal keratokonjonktivitte gözya makrofaj migrasyon inhibitör faktör düzeyleri.

    Species:
    Human
    Sample Type:
    Tears
    Year:
    2013

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