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> Human iPSCs From Fibroblasts (Normal, Male, African American, Neonate, NIST Line) (Commercial use)

Human iPSCs From Fibroblasts (Normal, Male, African American, Neonate, NIST Line) (Commercial use)

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Cat# ASE-9211

Size : 1vial(0.5x10^6)

Brand : Applied StemCell

Contact local distributor :


Phone : +1 850 650 7790

Human iPSCs From Fibroblasts (Normal, Male, African American, Neonate, NIST Line)

Human iPSCs From Fibroblasts (Normal, Male, African American, Neonate, NIST Line)

SKU ASE-9211 Categories iPS Cell Lines, iPSC Products, Products

Human iPSCs from Fibroblasts

Normal, Male, African American, Neonatal

Start your research with high quality human induced pluripotent stem cells (hiPSCs)

  • Ready-to-use iPSCs from Applied StemCell accelerate your studies with quality reagents
  • Episomal reprogramming and morphology-based selection preserves purity by reducing sources of heterogeneity
  • Reprogrammed from fibroblasts from a male, African American donor
  • Pluripotency confirmed through OCT4, SOX2, SSEA4, TRA-1-60, and TRA-1-81, and alkaline phosphatase (AP)

Overview

Applied StemCell offers high-quality hiPSCs (ASE-9211), generated from normal human skin fibroblasts using episomal reprogramming methods. This transient reprogramming approach utilizes human transcription factors (OCT4, SOX2, KLF4, and c-MYC) to initiate the reprogramming process without integrating foreign DNA into the genome. The iPSCs are selected based on precise morphological criteria, eliminating the need for fluorescent markers or drug-based selection, ensuring their purity and natural state.   Each order of ASE-9211 contains one vial of 0.5 x 106 cells. 

How it works

The ASE–9211 cell line is user-friendly, offering a seamless blend of safety, flexibility, and reliability.

1. Thaw and expand ASE-9211. 
  • Prepare Matrigel® or Geltrex-coated plates and thaw mTeSR-plus medium with Rock Inhibitor and Primocin.
  • Plate the cells into prepared wells and evenly distribute clumps for uniform growth.
  • Monitor colonies for attachment and stability; change the medium as needed.
2. Passaging human iPSCs 
  • Detach cells using a 0.5 mM EDTA solution.  
  • Replate cells into freshly coated wells, ensuring colonies remain intact. 
  • Incubate under appropriate conditions. 
3. Cryopreserving human iPSCs 
  • Detach colonies with EDTA and resuspend them in freezing medium. 
  • Transfer the suspension to labeled cryovials and store at -80°C overnight in a CoolCell® Freezing Container. Move cryovials to liquid nitrogen the next day for long-term storage. 

Can’t find the right iPSCs for your research? We offer custom reprogramming services to meet your specific needs.

ASE-9211 shows all pluripotency markers

Figure 1: We performed immunostaining with pluripotency markers OCT-4, SOX2, SSEA-4, TRA-1-60 and TRA-1-81(Top Row) and DAPI (Bottom row).

ASE-9211 can differentiate into 3 lineages

Figure 2: We performed immunostaining of hiPSC to confirm lineage commitment using nuclear marker DAPI (blue) Mesoderm (ME) marker: GATA4 (green); Ectoderm (EC) marker: β-Tubulin (red).

View the datasheet

Frequently asked questions

Didn’t find all the info you were looking for? 

Still have questions? 

No problem.

Speak with an expert

Yes, our ASE–9211 iPSC line is optimized for CRISPR/Cas9 editing, making it ideal for genetic studies and functional genomics.

We provide detailed protocols for thawing, feeder-free culturing, and cryopreservation, ensuring ease of use for your research team.

Each batch is tested for pluripotency markers (OCT4, SOX2, SSEA4, TRA-1-60, TRA-1-81) and alkaline phosphatase (AP) activity, alongside verification of a normal male karyotype to ensure purity and stability.

Each vial of the product is at P17 with 0.5 million cells / vial.

Ready to learn more?

Fill out the contact form below and a team member will be in touch within one business day.

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Cat#
Description
Cond.
Price Bef. VAT
2310-SC
Human Dermal Fibroblasts-neonatal
 5x10^5cells/vial