Human IgG Antibody - HRP Conjugated

Cat# OARA04964

Size : 1mg

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Goat Anti-Human IgG (gamma chain) Polyclonal Antibody - HRP Conjugated (OARA04964)

Product Info
Predicted Species ReactivityHuman
Product FormatLyophilized. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
ClonalityPolyclonal
IsotypeIgG
HostGoat
ConjugationHRP
ApplicationELISA|IHC|WB
Reconstitution and Storage-20°C or -80°C|2°C to 8°C
ImmunogenHuman IgG gamma heavy chain
PurificationThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Concentration1 mg/ml
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG and Human Serum. Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes.
Application InfoELISA: 1:10,000~100,000
WB: 1:500~1,000
IHC: 1:2,000~10,000
Optimal dilutions should be determined by the end user.
Publications

Immune response to SARS-CoV-2 variants after immunization with different vaccines in Mexico

Authors: Garay, E., Whelan, S. P. J., et al.

Journal: Epidemiol Infect. 152, e30 (2024)

Description of TargetAnti-Human IgG (gamma chain) Peroxidase generated in goat detects human Immunoglobulin G (gamma chain). It is a protein complex composed of four peptide chain - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75% of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Human IgG (gamma chain) Antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.
Molecular Weight40 kDa