| Datasheets/Manuals | Printable datasheet for Goat Anti-Human IgA (alpha chain) Polyclonal Antibody - AP Conjugated (OARA04656) |
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| Predicted Species Reactivity | Human |
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| Product Format | Liquid. 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 |
| Clonality | Polyclonal |
| Isotype | IgA |
| Host | Goat |
| Conjugation | AP |
| Application | DB|ELISA|IHC|WB |
| Additional Information | Conjugation Chemistry: Glutaraldehyde coupling |
| Reconstitution and Storage | 2°C to 8°C |
| Immunogen | Human IgA alpha heavy chain |
| Purification | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. |
| Concentration | 1 mg/ml |
| Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Human IgA and Human Serum. No reaction was observed against Human IgG F(c). Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes. |
| Application Info | This product has been assayed against 1.0 ug of Human IgA in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:7,000 is suggested for this product. ELISA: 1:2000~10000 IHC: 1:200~1000 WB: 1:500~2500 |
| Alias Symbols | goat anti-Human IgA (alpha chain) alkaline phosphatase conjugated Antibody, goat anti-Human IgA alpha Antibody alkaline phosphatase conjugation |
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| Description of Target | Anti-Human IgA Alkaline Phosphatase Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. |
| Molecular Weight | 140 kDa |