Human CD74 Antibody : LE/AF
Cat# OASB01158
Size : 0.5mg
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
Human CD74 Antibody : LE/AF (OASB01158)
| Datasheets/Manuals | Printable datasheet for Human CD74 Antibody : LE/AF (OASB01158) |
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| Tested Species Reactivity | Human |
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| Predicted Species Reactivity | Human |
| Clonality | Monoclonal |
| Clone | LN2 |
| Isotype | IgG1 |
| Host | Mouse |
| Conjugation | LE: Low Endotoxin, AF: Azide Free |
| Application | FC, IHC-F, IHC-P, ICC, IP, WB, BL |
| Additional Information | Description: CD74 represents a type II integral membrane protein known as MHC class II-associated invariant chain. It is moderately expressed on B cells and weakly on monocytes and macrophages. CD74 is also found on a subset of activated T cells, Langerhans cells, dendritic cells, and some epithelial cells. CD74 may play a role in antigen processing and presentation through intracellular transport and expression of class II molecules on the cell surface. |
| Reconstitution and Storage | Store at -20C |
| Immunogen | Cell line SU-DHL-4 nuclei |
| Concentration | 0.5 mg/mL |
| Specificity | CD74 |
| Characterization | To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform tocharacteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes,or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Purified (UNLB) antibody Fluorescein conjugate Biotin conjugate £ 1 mg/106 cells 10 mL/106 cells 10 mL/106 cells |
| Application Info | Flow cytometry, Immunohistochemistry (frozen sections and paraffin sections), Immunoprecipitation |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | · The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.0. No preservatives or amine- containing buffer salts added. Store at 2- 8 C · The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2- 8 C · The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2- 8 C · The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0mL of PBS. Aliquot and store at or below - 20 C · Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored asdirected. |
| Reference | 1. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: WhiteCell Differentiation Antigens, Oxford University Press, Oxford 2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte AntigensFacts Book, 2nd Edition, CD74 Section, Academic Press, New York, p. 327 3. Lamb, C.A., J.W. Yewdell, J.R. Bennink, and P. Cresswell. 1991. Proc. Natl. Acad. Sci. USA 88:5998 4. Viville, S., J. Neefjes, V. Lotteau, A. Dierich, M. Lemeur, H. Ploegh, C. Benoist, and D. Mathis. 1993. Cell 72:635. |
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| Gene Full Name | HLA class II histocompatibility antigen gamma chain |
| Alias Symbols | II, p33, DHLAG, HLADG, Ia-GAMMA |
| NCBI Gene Id | 972 |
| Uniprot ID | P04233 |
| Protein Accession # | NP_001020329.1 |
| Nucleotide Accession # | NM_001025158.2 |
