Human CD40b Antibody : LE/AF
Cat# OASB01095
Size : 0.5mg
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
Human CD40b Antibody : LE/AF (OASB01095)
| Datasheets/Manuals | Printable datasheet for Human CD40b Antibody : LE/AF (OASB01095) |
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| Tested Species Reactivity | Human |
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| Predicted Species Reactivity | Human |
| Clonality | Monoclonal |
| Clone | MM2/174 |
| Isotype | IgG1 |
| Host | Mouse |
| Conjugation | LE: Low Endotoxin, AF: Azide Free |
| Application | FC, IHC-F, IHC-P, ICC, IP, WB |
| Additional Information | Description: CD42b, also known as GPIb alpha, is a two-chain membrane glycoprotein that forms a noncovalent complex with CD42a (GPIX) on the surface of platelets and megakaryocytes. Binding of the GPIb/GPIX heterodimer to von Willebrand factor bound to exposed subendothelial surfaces is essential for platelet adhesion at sites of injury. |
| Reconstitution and Storage | Store at -20C |
| Immunogen | Human plasma membrane |
| Concentration | 0.5 mg/mL |
| Specificity | CD42b |
| Characterization | To insure lot- to- lot consistency, each batch of product is tested by flow cytometry to conform tocharacteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Purified (UNLB) antibody Fluorescein conjugate Biotin conjugate R-phycoerythrin conjugate Allophycocyanin conjugate Spectral Red conjugate <= 1 ug/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells |
| Application Info | Flow cytometry, Immunohistochemistry (frozen sections and paraffin sections), Western blotting |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.0. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent Store at 2- 8 C - The R- phycoerythrin (R- PE) is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent Store at 2- 8 C. Do not freeze! - The allophycocyanin (APC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and astabilizing agent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and astabilizing agent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored asdirected. |
| Reference | 1. Schlossman, S., L. Bloumsell, W. Gilks, J.M. Harlan, C. Kishimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd,eds. 1995. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, Oxford 2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte AntigensFacts Book, 2nd Edition, CD42a, b Section, Academic Press, New York, p. 235 3. Thorsen, L.I., G. Gaudernack, N. Hack, J.M. Wilkinson, F. Brosstad, N.O. Solum, and N. Crawford. 1988. Brit. J. Haematol. 68:67 4. Hornby, E.J., S. Brown, J.M. Wilkinson, C. Mattock, and K.S. Authi. 1991. Brit. J. Haematol. 79:277 5. Berndt, M.C., X.P. Du, and W.J. Booth. 1987. Biochemistry 27:633 6. Roth, G.J. 1992. Immunol. Today 13:100. |
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| Gene Symbol | GP1BA |
| Gene Full Name | Platelet glycoprotein Ib alpha chain |
| Alias Symbols | BSS, GP1B, VWDP, CD42B, GPIbA, BDPLT1, BDPLT3, DBPLT3, GPIbalpha, CD42b-alpha |
| NCBI Gene Id | 2811 |
| Protein Name | platelet glycoprotein Ib alpha chain |
| Description of Target | Glycoprotein Ib (GP Ib) is a platelet surface membrane glycoprotein composed of a heterodimer, an alpha chain and a beta chain, that is linked by disulfide bonds. The Gp Ib functions as a receptor for von Willebrand factor (VWF). The complete receptor complex includes noncovalent association of the alpha and beta subunits with platelet glycoprotein IX and platelet glycoprotein V. The binding of the GP Ib-IX-V complex to VWF facilitates initial platelet adhesion to vascular subendothelium after vascular injury, and also initiates signaling events within the platelet that lead to enhanced platelet activation, thrombosis, and hemostasis. This gene encodes the alpha subunit. Mutations in this gene result in Bernard-Soulier syndromes and platelet-type von Willebrand disease. The coding region of this gene is known to contain a polymophic variable number tandem repeat (VNTR) domain that is associated with susceptibility to nonarteritic anterior ischemic optic neuropathy. |
| Uniprot ID | P07359 |
| Protein Accession # | NP_000164.5 |
| Nucleotide Accession # | NM_000173.6 |
