RayBio® C-Series Human Autophagy Array 1 Kit. Detects 20 Human Autophagic Factors. Suitable for all liquid sample types.
Human Autophagy Array C1
Human Autophagy Array C1
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Accession Number | O75582|Q16832|P11279|Q9H492|O95352|O94817|Q14457|Q9Y4P1|Q9H1Y0|Q9GZQ8|Q15382|O60934|Q9H0Y0|Q9NT62|Q8WYN0|O95166|O75143|Q13501|P37840|O60238 |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 20 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
| ATG12
| ATG7
| ATG10
| ATG13
| ATG3
|
| ATG4A
| ATG4B
| ATG5
| Beclin
| BNIP3L
|
| DDR2
| GABARAP
| LAMP1 (CD107a)
| LC3a
| LC3B
|
| NBS1
| P62
| RHEB
| MSK1
| Alpha-synuclein
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Autophagy Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
Autophagy and Barrier Disruption in Patients with Complex Regional Pain Syndrome
- Species:
- Human
- Sample Type:
- Plasma (CRPS patients vs fracture controls)
- Year:
- 2026
Unravelling inclusion body myositis using a patient-derived fibroblast model
- Species:
- Human
- Sample Type:
- fibroblasts
- Year:
- 2023
- Journal Name:
- Journal of Cachexia Sarcopenia and Muscle
HS 3D-SeboSkin Model Enables the Preclinical Exploration of Therapeutic Candidates for Hidradenitis Suppurativa/Acne Inversa
- Species:
- Human
- Sample Type:
- skin
- Year:
- 2023
- Journal Name:
- Pharmaceutics
Keap1 inhibition sensitizes head and neck squamous cell carcinoma cells to ionizing radiation via impaired non-homologous end joining and induced autophagy
- Species:
- Human
- Year:
- 2020
- Journal Name:
- Cell Death & Disease
Spermidine-Eugenol Supplement Preserved Inflammation-Challenged Intestinal Cells by Stimulating Autophagy
- Species:
- Human
- Sample Type:
- NCM460 cell
- Year:
- 2023
- Journal Name:
- International Journal of Molecular Sciences
Wheat Germ Spermidine and Clove Eugenol in Combination Stimulate Autophagy In Vitro Showing Potential in Supporting the Immune System against Viral Infections
- Year:
- 2022
- Journal Name:
- Molecules
- PubMed ID:
- 35684363
Doxorubicin-induced normal breast epithelial cellular aging and its related breast cancer growth through mitochondrial autophagy and oxidative stress mitigated by ginsenoside Rh2
- Species:
- Human
- Sample Type:
- Conditioned Media (cell culture media)
- Year:
- 2020
- Journal Name:
- Phytotherapy Research
Molecular evidence of autophagy impairment as serum level of ATG13 is elevated in patients with myalgic encephalomyelitis and chronic fatigue syndrome: two independent case-control studies
- Species:
- Human
- Sample Type:
- Serum (ME/CFS patients and controls)
- Year:
- 2021
- Journal Name:
- Research Square
Stable silencing of ROR1 regulates cell cycle, apoptosis, and autophagy in a lung adenocarcinoma cell line
- Species:
- Human
- Sample Type:
- Cell Lysate (lung adenocarcinoma)
- Year:
- 2020
- Journal Name:
- International Journal of Clinical and Experimental Pathology
Positively Correlated CD47 Activation and Autophagy in Umbilical Cord Blood-Derived Mesenchymal Stem Cells during Senescence
- Species:
- Human
- Sample Type:
- Cell Lysate (cell lysates of UCB-MSCs); Tissue Lysate / Homogenate
- Year:
- 2021
- Journal Name:
- Stem Cells International
- PubMed ID:
- 33936211