HMOX1 ELISA Kit (Chicken)
Cat# OKEH06597
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
HMOX1 ELISA Kit (Chicken) (OKEH06597)
| Datasheets/Manuals | Printable datasheet for HMOX1 ELISA Kit (Chicken) (OKEH06597) |
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| Predicted Species Reactivity | Chicken|Gallus gallus | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Hmox1 ELISA Kit (Chicken) (OKEH06597) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hmox1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hmox1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hmox1 captured in the well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.78-50ng/ml | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates. Intra-Assay CV <10% Inter-Assay CV <12% | ||||||||||||||||||||||
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates | ||||||||||||||||||||||
| Sensitivity | 0.19 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Chicken Heme oxygenase 1 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | HMOX1 |
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| Gene Full Name | heme oxygenase 1 |
| Alias Symbols | heme oxygenase (decycling) 1, heme oxygenase 1, HO-1. |
| NCBI Gene Id | 396287 |
| Protein Name | Heme oxygenase 1 |
| Description of Target | Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. |
| Uniprot ID | P14791 |
| Protein Accession # | NP_990675 |
| Nucleotide Accession # | NM_205344 |
