Reagents and instruments for immunology, cell biology and molecular biology.
 
> hCD70-muCD8 Fusion Protein (human CD70-murine CD8 Fusion Protein)

hCD70-muCD8 Fusion Protein (human CD70-murine CD8 Fusion Protein)

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Cat# C2425-01Q-25ug

Size : 25ug

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790

C2425-01Q hCD70-muCD8 Fusion Protein (human CD70-murine CD8 Fusion Protein)

Grade
Purified
Applications
E FC
Shipping Temp
Blue Ice
Storage Temp
-20°C

Human CD70 (TNFSF7) is the ligand for CD27 (TNFRSF7) and is notably absent on resting lymphocytes. CD70 is expressed after activation on about 20% of T cells and on about 70% of B cells. Recombinant CD70-muCD8 binds to cell surface CD27 on human peripheral blood leukocytes.

Molecular Structure:  A soluble molecule consisting of the extracellular domain (167aa) of murine CD8 alpha fused to 146 (c-term) amino acids of the extracellular domain of human CD70, with a predicted monomeric weight of 34.8kD.

Applications:
Suitable for use in ELISA and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Purity: Purified by size exclusion.|Concentration: ~0.5mg/ml|Form: Supplied as liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.5mg/ml gentamicin sulfate.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Human CD70 expressed in CHO cells.
Form
Supplied as liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.5mg/ml gentamicin sulfate.
Purity
Purified by size exclusion.
References
1. R.Q. Hintzen, et al, (1994) J Immunol 152: 1762-1773, 2. M.R. Bowman, et al, (1994) J Immunol 152: 1756-1761, 3. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1137-1138, 4. K. Agematsu, et al, (1995) J Immunol 154: 3627-3635, 5. T. F. Rowley , A Al-Shamkhani, (2004) J Immunol 172: 6039-6046, 6. J. M.Carr, D. T. Fearon, et al. (2006) PNAS:103(51):19454-19459