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> Guinea pig IL-12p40(Interleukin 12 p40) ELISA Kit

Guinea pig IL-12p40(Interleukin 12 p40) ELISA Kit

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Cat# NB-66-26905-48T

Size : 48T

Brand : Neo Biotech

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Phone : +1 850 650 7790

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Guinea pig IL-12p40(Interleukin 12 p40) ELISA Kit
One-click to copy product information
ELK5872
Size： ：
48 T
96 T
96 T*5
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Overview

Product name: Guinea pig IL-12p40(Interleukin 12 p40) ELISA Kit
Reactivity: Guinea pig
Alternative Names: IL-12B; p40; IL12B; CLMF2; NKSF2; Natural Killer Cell Stimulatory Factor 2; Cytotoxic Lymphocyte Maturation Factor 2; Interleukin 23, P40 Subunit
Assay Type: Sandwich
Sensitivity: 3 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length: 3.5h
Research Area: Cytokine;Infection immunity;Immune molecule;
Uniprot ID: Q924V5
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig IL-12p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig IL-12p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig IL-12p40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig IL-12p40 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
500.00 2.054 1.981
250.00 1.633 1.560
125.00 1.139 1.066
62.50 0.882 0.809
31.25 0.518 0.445
15.63 0.327 0.254
7.82 0.190 0.117
0.00 0.073 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IL-12p40 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-12p40 in samples.
Matrix Recovery range Average
serum(n=5) 79-93% 86%
EDTA plasma(n=5) 95-107% 101%
Heparin plasma(n=5) 85-95% 90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-12p40 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 93-104% 83-96% 95-103% 88-95%
EDTA plasma(n=5) 88-97% 91-103% 86-92% 89-97%
Heparin plasma(n=5) 85-94% 87-96% 89-101% 82-90%