Granulocyte Macrophage Colony Stimulating Factor, Recombinant, Human, Animal Free (GM-CSF, Granulocyte/Macrophage Colony-Stimulating Factor, CSF- 2, MGI-1GM, Pluripoietin-a)

Cat# 208626-50ug

Size : 50ug

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790


208626 Granulocyte Macrophage Colony Stimulating Factor, Recombinant, Human, Animal Free (GM-CSF, Granulocyte/Macrophage Colony-Stimulating Factor, CSF- 2, MGI-1GM, Pluripoietin-a)

Grade
Highly Purified
Accession #
NP_034099
Shipping Temp
Blue Ice
Storage Temp
-20°C

GM-CSF is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors shows significant homologies with other receptors for hematopoietic growth factors, including IL2-beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors. Recombinant Human GM-CSF is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14477D.

Amino Acid Sequence:
MAPARSPSP STQPWEHVN AIQEARRLL NLSRDTAAE MNETVEVIS EMFDLQEPT CLQTRLELY KQGLRGSLT KLKGPLTMM ASHYKQHCP PTPETSCAT QIITFESFK ENLKDFLLV IPFDCWEPV QE

Biological Activity:
The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤0.1 ng/ml, corresponding to a specific activity of ≥1x10e7units/mg.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications
Source: Recombinant, E. coli|Purity: ≥98% by HPLC and SDS-PAGE. Endotoxin: ≤0.1ng/ug (IEU/ug)|Form: Supplied as a lyophilized powder in pH 7.2, 10mM sodium citrate. Reconstitute with sterile ddH2O to 0.1-1mg/ml||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a lyophilized powder in pH 7.2, 10mM sodium citrate. Reconstitute with sterile ddH2O to 0.1-1mg/ml
Purity
≥98% by HPLC and SDS-PAGE. Endotoxin: ≤0.1ng/ug (IEU/ug)