Product Name :
Goat Anti-Rabbit IgG (H&L) Highly Cross-Adsorbed - AF488 Host :
Goat Application :
E, IH, IF, FC Classification :
Secondary Antibodies Immunogen :
Rabbit IgG Label :
AF488 Concentration :
1 mg/ml Suggested Dilution Range :
IH (1/100 - 1/1000), IF (1/100 - 1/1000), FC (1/1000 - 1/4000), E (Use at an assay dependent concentration) Description :
Goat Polyclonal Secondary Antibody to Rabbit IgG (H&L) Highly Cross-Adsorbed and conjugate to AF488 Buffer :
1 mg/ml, liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide Preservative :
0.02% Proclin/Sodium Azide Purification&Purity :
Goat Polyclonal Secondary Antibody to Rabbit IgG (H&L) have been cross-adsorbed against IgG from bovine, goat, guinea pig, hamster, horse, mouse, rat, and human. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins. Storage&Stability :
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. Expiration Date :
Six months from date of reconstitution. Background :
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG. No antibody was detected against non immunoglobulin serum proteins.

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Blocking peptide available as BS22352P