Glutathione (GSH) Colorimetric Detection BioAssay™ Kit
Cat# 474023-4plates
Size : 4plates
Brand : US Biological
Contact local distributor :
Phone : +1 850 650 7790
474023 Glutathione (GSH) Colorimetric Detection BioAssay™ Kit
Shipping Temp
Blue IceStorage Temp
4°C/-20°C
Sample Type:
Whole blood, serum, plasma, erythrocytes, urine, cell lysates, and tissue samples
Target:
Glutathione and Oxidized Glutathione
Method of Analysis:
Absorbance Plate Reader
Intended Use:
Quantitatively measure glutathione (GSH) and oxidized glutathione (GSSG) present in a variety of samples, including whole blood, serum, plasma, erythrocytes, urine, cell lysates, and tissue samples. Analyze the absorbance colorimetric signal by spectrophotometer.
Test Principle:
Glutathione, gamma-L-glutamyl-L-cysteinyl-glycine or GSH, is the most abundant non-protein thiol in cells. GSH is involved in the regulation of a number of essential biochemical processes within the cell. Primarily recognized as a key intracellular source of reducing power for combating the toxic accumulation of free radical byproducts, GSH is also involved with detoxifying and removal of exo/endogenous toxins and alkylating agents. In its role as a cell signaling agent, GSH is involved in DNA synthesis and cell proliferation regulation. Due to the genetically conserved molecular processes by which cells die, intracellular levels of GSH can favor a cell death pathway via apoptosis (adequate intracellular GSH stores) or via necrosis or autophagy (depleted intracellular GSH stores). Detection of a drop in intracellular GSH concentration in an experimental cell population relative to a negative (healthy cell) control is often indicative of an apoptosis induction event. Glutathione Colorimetric Detection BioAssay™ Kit is designed to quantitatively measure GSH and GSSG in a variety of samples.
Kit Components:
4 clear half-area polystyrene 96-well microtiter plates
Oxidized Glutathione (GSSG) Standard, 1x250uM, 1x350uL
Colorimetric GSH Detection Substrate, 1x1mL
Glutathione Assay Buffer, 1x225mL
NADPH Concentrate, 1x1mL
Glutathione Reductase Concentrate, 1x1mL
Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.