Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with GLI3 antibody (GTX104362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GLI3 antibody detects GLI3 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
GLI3 antibody (GTX104362) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GLI3(GTX104362) antibody at 1:500 dilution.

The data was published in the journal Cell Commun Signal in 2014. PMID: 24548465

The data was published in the journal Elife in 2016. PMID: 27185526