Formate Colorimetric Assay Kit
Cat# E-BC-K1100-M-96T
Size : 96T
Brand : Elabscience
Contact local distributor :
Phone : +1 850 650 7790
| Detection Principle | Formic acid dehydrogenase (FDH) catalyzes the reaction of formic acid with NAD+ to produce NADH. NADH, under the action of PMS, transfers electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. The NAD(P)H in the sample itself will cause certain background interference, so set the control well in the measurement process to eliminate such interference. |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (450 nm) |
| Research Area | Other |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 8.20 μmol/L |
| Detection Range | 8.20-800 μmol/L |
| Precision | inter-assay CV: 3.9% | intra-assay CV: 3.3% |
| Sample Volume | 50 μL |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Dog serum | 1 |
| Mouse serum | 1 |
| Horse serum | 1 |
| Porcine serum | 1 |
| Rat plasma | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat spleen tissue homogenate | 1 |
| 10% Rat brain tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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