FN-Click EdU Cell Proliferation Flow Cytometry Assay Kit (Green, FineTest®488)

FNCK107

50T/200T

Note: 50 T means that 50 samples can be tested with 6 well plates. EdU (10 mM) needs to be stored in aliquots for the first use(50 μL/ vial is recommended or aliquot into smaller quantities according to experimental needs).

Store at -20°C for 1 year.FineTest®488 Azide I needs to be stored away from light.

FN-Click EdU Cell Proliferation Flow Cytometry Assay kit (green, FineTest®488) is convenient and sensitive for proliferation detection of cell suspension samples. The results can be analyzed by flow cytometry.

Cell proliferation detection is widely used in the evaluation of cell activity, genotoxicity and efficacy of anti-tumor drugs. Direct detection of DNA synthesis in cells is considered to be the most accurate method to detect cell proliferation.The first widely used method to detect DNA synthesis in cells was the radionuclide incorporation method, but this method was greatly limited due to radioactive contamination and the difficulty of single-cell detection, and it was gradually replaced by the BrdU method based on antibody detection. BrdU method has many steps and requires the use of BrdU antibody, which has many influencing factors and poor stability.

EdU method is based on EdU incorporation and subsequent click reaction, without the use of antibodies, convenient operation, high detection sensitivity, is a new method to upgrade on the basis of BrdU method, will gradually replace BrdU method. EdU(5-ethynyl-2-deoxyuridine) is a thymine deoxyriboside analogue that can be incorporated into newly synthesized DNA in place of thymine deoxyriboside during DNA synthesis. On the other hand, the acetylene group on EdU can covalently react with a fluorescently labeled small molecule azide probe to form a stable triazole ring catalyzed by a monovalent copper ion, which is a very rapid reaction known as the Click reaction.Through the click reaction, the newly synthesized DNA is labeled with a corresponding fluorescent probe, so that the proliferating cells can be detected using appropriate fluorescence detection equipment.

Test results refer to the figure below: