Fish Insulin,INS ELISA Kit
Cat# CSB-E12123Fh-96T
Size : 96T
Brand : Cusabio
Contact local distributor :
Phone : +1 850 650 7790
| Size | 96T,5×96T,10×96T |
Product Details
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of fish INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-100 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 80-89 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 83-99 | |||||
| The recovery of fish INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 88-98 | ||||
| EDTA plasma (n=4) | 92 | 90-95 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||
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and FAQs
This Fish INS ELISA Kit was designed for the quantitative measurement of Fish INS protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 100 pg/mL-1600 pg/mL and the sensitivity is 100 pg/mL.
Citations
- Q Wang, H Ge, Z Gu, H Chen, H Mu, K Mai, W Zhang,Metabolites,2026
- X Zhi, Y Gu, W Zhao, S Xie, Y Bao, S Morais, D Li,Aquaculture Reports,2025
- R Shao,The Journal of nutritional biochemistry,2024
- R Shao,FASEB journal,2023
- WX Li,Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology,2023
- Z Zhao,bioRxiv,2023
- HyojinLee,Journal of Hazardous Materials,2022
- XC Yuan,Fish Physiol Biochem,2021
- Ye G, et al,Aquaculture Research,2019
- Wang Q,Fish Physiology and Biochemistry,2019
Customer Reviews and Q&A
There are currently no reviews for this product.
Please provide some more information about the following.
a. capture antibody or capture antigen
b. Detection antibody (if applicable)
c. standard – if recombinant protein please specify sequence (including species source and accession no. if available) and expression host.
The customer would like to use this assay to test islet cell samples in zebrafish. Have you tested tissue homogenate samples wit this assay, if yes please provide the reference data. Can you recommend a dilution for the customer’s samples.
CSB-E12123Fh
This is a competitive kit.
The plate is coated with mouse monoclonal antibody.
Standard is CHO-derived recombinant protein.
Pls find the manual as attached by e-mail.
We suggest you do a pretest first if you want to detect tissue samples. You can use 1*PBS as diluent.Pls let me know if you have any further questions. Thank you.
I would like to look for ELISA kits for insulin levels in zebrafish. What would you recommend?