FBLN5 ELISA Kit (Rat)
Cat# OKEH06327
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
FBLN5 ELISA Kit (Rat) (OKEH06327)
| Datasheets/Manuals | Printable datasheet for FBLN5 ELISA Kit (Rat) (OKEH06327) |
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| COA Datasheet | Printable COA datasheet for OKEH06327 |
| Predicted Species Reactivity | Rat|Rattus norvegicus | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Fbln5 ELISA Kit (Rat) (OKEH06327) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fbln5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Fbln5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fbln5 captured in the well. | ||||||||||||||||||||||
| ELISA Kit Range | 3.12-200ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 106% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision. Mean Intra-Assay Precision - <5.2%, n = 20 Mean Inter-Assay Precision - <8.0%, n = 20 | ||||||||||||||||||||||
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 1.56 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Rat Fibulin-5 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||||||||
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| Gene Symbol | Fbln5 |
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| Gene Full Name | fibulin 5 |
| Alias Symbols | dance, developmental arteries and neural crest EGF-like protein, embryonic vascular EGF repeat-containing protein, EVEC, FIBL-5, fibulin-5. |
| NCBI Gene Id | 29158 |
| Protein Name | Fibulin-5 |
| Description of Target | Essential for elastic fiber formation, is involved in the assembly of continuous elastin (ELN) polymer and promotes the interaction of microfibrils and ELN. Stabilizes and organizes elastic fibers in the skin, lung and vasculature. Promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. Vascular ligand for integrin receptors which may play a role in vascular development and remodeling. May act as an adapter that mediates the interaction between FBN1 and ELN. |
| Uniprot ID | Q9WVH8 |
| Protein Accession # | NP_062026.2 |
| Nucleotide Accession # | NM_019153.3 |
| Protein Size (# AA) | 448 |
