Quimigen Portugal > ERK1/2 (phospho-T202/Y204) polyclonal antibody
ERK1/2 (phospho-T202/Y204) polyclonal antibody
Cat# BS5016
Size : 100ul
Contact local distributor :
Phone : +1 850 650 7790
Brand : Bioworld Technology
Contact local distributor :
Phone : +1 850 650 7790
| Product Name | ERK1/2 (phospho-T202/Y204) polyclonal antibody |
| Catalog No. | BS5016 |
| Swiss-Prot | P27361/P28482 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB IHC |
| Application_all | WB: 1:500~1:1000 IHC: 1:50~1:200 |
| BiowMW | ~ 42,44 kDa |
| Alternative Name | Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p44-ERK1; MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2; MAPK1; ERK2; PRKM1; PRKM2 |
| Purification&Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
| Conjugate | Unconjugated |
| Modification | Phosphorylation |
Datasheet
COA
MSDS
SHIP Product Name :
ERK1/2 (phospho-T202/Y204) polyclonal antibody Background :
The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK 1 and Threonine 185 and Tyrosine 187 residues of ERK 2 is required for full enzymatic activation. The structural consequences of dual phosphorylation in ERK 2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-ERK1/2 (T202/Y204) polyclonal antibody detects endogenous levels of ERK1 protein when phosphorylated at threonine 202 and tyrosine 204, and ERK2 protein when phosphorylated at threonine 185 and tyrosine 187. Immunogen :
Synthetic phosphopeptide derived from human ERK1/2 around the phosphorylation site of Threonine 202/Tyrosine 204. Conjugate :
Unconjugated Modification :
Phosphorylation
ERK1/2 (phospho-T202/Y204) polyclonal antibody Background :
The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK 1 and Threonine 185 and Tyrosine 187 residues of ERK 2 is required for full enzymatic activation. The structural consequences of dual phosphorylation in ERK 2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-ERK1/2 (T202/Y204) polyclonal antibody detects endogenous levels of ERK1 protein when phosphorylated at threonine 202 and tyrosine 204, and ERK2 protein when phosphorylated at threonine 185 and tyrosine 187. Immunogen :
Synthetic phosphopeptide derived from human ERK1/2 around the phosphorylation site of Threonine 202/Tyrosine 204. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of p-ERK1/2 (T202/Y204) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate treated with UV(4h) Lane2:Raw264.7 whole cell lysate treated with UV(4h) Lane3:PC12 whole cell lysate treated with UV(4h) - Immunohistochemistry (IHC) analyzes of p-ERK1/2 (T202/Y204) pAb in paraffin-embedded human breast carcinoma tissue.
Regulator of G‐protein signalling 5 protects against atherosclerosis in apolipoprotein E‐deficient mice PMCID: Pubmed No.:25363362 PTP1B promotes cell proliferation and metastasis through activating src and ERK1/2 in non-small cell lung cancer PMCID: Pubmed No.:25617799 Regulator of G protein signaling 5 protects against cardiac hypertrophy and fibrosis during biomechanical stress of pressure overload PMCID: Pubmed No.:20643937 Lipoxin A 4 Inhibits Transition of Epithelial to Mesenchymal Cells in Proximal Tubules PMCID: Pubmed No.:20714129 Endothelial cells dysfunction induced by silica nanoparticles through oxidative stress via JNK/P53 and NF-kB pathways PMCID: Pubmed No.:20727582 HOXB7 as a prognostic factor and mediator of colorectal cancer progression PMCID: Pubmed No.:21474578 Isomalto oligosaccharide sulfate inhibits tumor growth and metastasis of hepatocellular carcinoma in nude mice PMCID: Pubmed No.:21513518 Hippocampal Neuronal Nitric Oxide Synthase Mediates the Stress-Related Depressive Behaviors of Glucocorticoids by Downregulating Glucocorticoid Receptor PMCID: Pubmed No.:21613472 XJP-1, a novel ACEI, with anti-inflammatory properties in HUVECs PMCID: Pubmed No.:21803355 Hydrogen Sulfide Protects against Chemical Hypoxia-Induced Injury by Inhibiting ROS-Activated ERK1/2 and p38MAPK Signaling Pathways in PC12 Cells PMCID: Pubmed No.:21998720 Ghrelin-Attenuated Cognitive Dysfunction in Streptozotocin-induced Diabetic Rats. PMCID: Pubmed No.:22005105 MiR-146a suppresses tumor growth and progression by targeting EGFR pathway and in a p-ERK-dependent manner in castration-resistant prostate cancer PMCID: Pubmed No.:22161865 Disruption of mindin exacerbates cardiac hypertrophy and fibrosis PMCID: Pubmed No.:22367478 Interplay between Cell Migration and Neurite Outgrowth Determines SH2B1b-Enhanced Neurite Regeneration of Differentiated PC12 Cells PMCID: Pubmed No.:22539954 MicroRNA-133b Inhibits the Growth of Non-small Cell Lung Cancer by Targeting the Epidermal Growth Factor Receptor PMCID: Pubmed No.:22883469 1-(2,6-Dibenzyloxybenzoyl)-3-(9H-fluoren-9-yl)-urea: A novel cyclophilin A allosteric activator PMCID: Pubmed No.:22906739 Osteogenesis and angiogenesis induced by porous β-CaSiO3/PDLGA composite scaffold via activation of AMPK/ERK1/2 and PI3K/Akt pathways PMCID: Pubmed No.:23069715 Effect of Heparin-Derived Oligosaccharide on Vascular Smooth Muscle Cell Proliferation and the Signal Transduction Mechanisms Involved PMCID: Pubmed No.:23097139 Dietary flavonoid genistein induces Nrf2 and phase II detoxification gene expression via ERKs and PKC pathways and protects against oxidative stress in Caco-2 cells PMCID: Pubmed No.:23255485 The viral oncogene Np9 acts as a critical molecular switch for co-activating β-catenin, ERK, Akt and Notch1 and promoting the growth of human leukemia stem/progenitor cells PMCID: Pubmed No.:23307033 Interferon regulatory factor 3 is a negative regulator of pathological cardiac hypertrophy PMCID: Pubmed No.:23307144 MicroRNA-31 activates the Ras pathway and functions as an oncogenic microRNA in human colorectal cancer by repressing RAS p21 GTPase activating protein 1 (RASA1) PMCID: Pubmed No.:23322774 Inhibitory effect of oleanolic acid on hepatocellular carcinoma via ERK-p53-mediated cell cycle arrest and mitochondrial-dependent apoptosis PMCID: Pubmed No.:23404993 Electromagnetic-pulse-induced activation of p38 MAPK pathway and disruption of blood–retinal barrier PMCID: Pubmed No.:23570913 miR-145 inhibits isoproterenol-induced cardiomyocyte hypertrophy by targeting the expression and localization of GATA6. PMCID: Pubmed No.:23624080 4-Hydroxybenzyl Alcohol Confers Neuroprotection Through Up-Regulation of Antioxidant Protein Expression. PMCID: Pubmed No.:23624876 S-nitrosylation of ERK inhibits ERK phosphorylation and induces apoptosis PMCID: Pubmed No.:23657295 Dihydrotestosterone alters urocortin levels in human umbilical vein endothelial cells. PMCID: Pubmed No.:23801677 Lipoxin A4-induced heme oxygenase-1 protects cardiomyocytes against hypoxia/reoxygenation injury via p38 MAPK activation and Nrf2/ARE complex. PMCID: Pubmed No.:23826208 The stimulation of osteogenic differentiation of mesenchymal stem cells and vascular endothelial growth factor secretion of endothelial cells by β-CaSiO3/β-Ca3(PO4)2 scaffolds PMCID: Pubmed No.:23894078 Protective effect of flavonoid-rich extract from Rosa laevigata Michx on cerebral ischemia–reperfusion injury through suppression of apoptosis and inflammation PMCID: Pubmed No.:24012531 Metallothionein 1G functions as a tumor suppressor in thyroid cancer through modulating the PI3K/Akt signaling pathway. PMCID: Pubmed No.:24098937 Sesquiterpene dimer (DSF-52) from Artemisia argyi inhibits microglia-mediated neuroinflammation via suppression of NF-κB, JNK/p38 MAPKs and Jak2/Stat3 signaling pathways PMCID: Pubmed No.:24055519 NF-κB, ERK, p38 MAPK and JNK contribute to the initiation and/or maintenance of mechanical allodynia induced by tumor necrosis factor-alpha in the red nucleus PMCID: Pubmed No.:24161765 Cardioprotection of Vitexin on Myocardial Ischemia/Reperfusion Injury in Rat via Regulating Inflammatory Cytokines and MAPK Pathway PMCID: Pubmed No.:24228599 An Apple Oligogalactan Potentiates the Growth Inhibitory Effect of Celecoxib on Colorectal Cancer PMCID: Pubmed No.:24274457 Cuprous oxide nanoparticles inhibit angiogenesis via down regulation of VEGFR2 expression PMCID: Pubmed No.:24499922 Potential Application of Hydrolyzed Fish Collagen for Inducing the Multidirectional Differentiation of Rat Bone Marrow Mesenchymal Stem Cells PMCID: Pubmed No.:24359018 Proteomic analysis of SP600125-controlled TrkA-dependent targets in SK-N-MC neuroblastoma cells: Inhibition of TrkA activity by SP600125 PMCID: Pubmed No.:24375967 ERK MAP kinase activation in spinal cord regulates phosphorylation of Cdk5 at serine 159 and contributes to peripheral inflammation induced pain/hypersensitivity. PMCID: Pubmed No.:24498195 GPER-mediated proliferation and estradiol production in breast cancer-associated fibroblasts. PMCID: Pubmed No.:24481325 Cytohesins/ARNO: The Function in Colorectal Cancer Cells PMCID: Pubmed No.:24618737 Dual Orexin Receptor Antagonist 12 Inhibits Expression of Proteins in Neurons and Glia Implicated in Peripheral and Central Sensitization PMCID: Pubmed No.:24685439 Long-term blue light exposure induces RGC-5 cell death in vitro: involvement of mitochondria-dependent apoptosis, oxidative stress, and MAPK signaling pathways PMCID: Pubmed No.:24682673 Effect of Tenuifoliside A isolated from Polygala tenuifolia on the ERK and PI3K pathways in C6 glioma cells. PMCID: Pubmed No.:24877714 Arctigenin but not arctiin acts as the major effective constituent of Arctium lappa L. fruit for attenuating colonic inflammatory response induced by dextran sulfate sodium in mice. (Application: Mouse WB) PMCID: Pubmed No.:25284342 The activation of G protein-coupled receptor 30 (GPR30) inhibits proliferation of estrogen receptor-negative breast cancer cells in vitro and in vivo. (Application: Human WB) PMCID: Pubmed No.:25275589 Regulator of G Protein Signalling 5 protects against atherosclerosis in apolipoprotein E-deficient mice. PMCID: Pubmed No.:25363362 Frequent amplification of PTP1B is associated with poor survival of gastric cancer patients PMCID: Pubmed No.:25590580 Nicotine stimulates expression of proteins implicated in peripheral and central sensitization PMCID: Pubmed No.:25637801 PTP1B promotes cell proliferation and metastasis through activating src and ERK1/2 in non-small cell lung cancer PMCID: Pubmed No.:25617799 The Red Nucleus TNF-α Participates in the Initiation and Maintenance of Neuropathic Pain Through Different Signaling Pathways PMCID: Pubmed No.:25952358 Arctigenin but not arctiin acts as the major effective constituent of Arctium lappa L. fruit for attenuating colonic inflammatory response induced by dextran sulfate sodium in mice. (Application: Mouse WB) PMCID: Pubmed No.:25284342 The activation of G protein-coupled receptor 30 (GPR30) inhibits proliferation of estrogen receptor-negative breast cancer cells in vitro and in vivo. (Application: Human WB) PMCID: Pubmed No.:25275589 Regulator of G Protein Signalling 5 protects against atherosclerosis in apolipoprotein E-deficient mice. PMCID: Pubmed No.:25363362 Metallothionein 1G functions as a tumor suppressor in thyroid cancer through modulating the PI3K/Akt signaling pathway. PMCID: Pubmed No.:24098937 Sesquiterpene dimer (DSF-52) from Artemisia argyi inhibits microglia-mediated neuroinflammation via suppression of NF-κB, JNK/p38 MAPKs and Jak2/Stat3 signaling pathways PMCID: Pubmed No.:24055519 NF-κB, ERK, p38 MAPK and JNK contribute to the initiation and/or maintenance of mechanical allodynia induced by tumor necrosis factor-alpha in the red nucleus PMCID: Pubmed No.:24161765 Cardioprotection of Vitexin on Myocardial Ischemia/Reperfusion Injury in Rat via Regulating Inflammatory Cytokines and MAPK Pathway PMCID: Pubmed No.:24228599 An Apple Oligogalactan Potentiates the Growth Inhibitory Effect of Celecoxib on Colorectal Cancer PMCID: Pubmed No.:24274457 Cuprous oxide nanoparticles inhibit angiogenesis via down regulation of VEGFR2 expression PMCID: Pubmed No.:24499922 Potential Application of Hydrolyzed Fish Collagen for Inducing the Multidirectional Differentiation of Rat Bone Marrow Mesenchymal Stem Cells PMCID: Pubmed No.:24359018 Proteomic analysis of SP600125-controlled TrkA-dependent targets in SK-N-MC neuroblastoma cells: Inhibition of TrkA activity by SP600125 PMCID: Pubmed No.:24375967 ERK MAP kinase activation in spinal cord regulates phosphorylation of Cdk5 at serine 159 and contributes to peripheral inflammation induced pain/hypersensitivity. PMCID: Pubmed No.:24498195 GPER-mediated proliferation and estradiol production in breast cancer-associated fibroblasts. PMCID: Pubmed No.:24481325 Cytohesins/ARNO: The Function in Colorectal Cancer Cells PMCID: Pubmed No.:24618737 Dual Orexin Receptor Antagonist 12 Inhibits Expression of Proteins in Neurons and Glia Implicated in Peripheral and Central Sensitization PMCID: Pubmed No.:24685439 Long-term blue light exposure induces RGC-5 cell death in vitro: involvement of mitochondria-dependent apoptosis, oxidative stress, and MAPK signaling pathways PMCID: Pubmed No.:24682673 provides growth and metastasis advantage for human hepatocellular carcinoma PMCID: Pubmed No.:28844099 Reg3α Overexpression Protects Pancreatic β Cells from Cytokine-Induced Damage and Improves Islet Transplant Outcome PMCID: Pubmed No.:25826674 The Involvement of Protease Nexin-1 (PN1) in the Pathogenesis of Intervertebral Disc (IVD) Degeneration PMCID: Pubmed No.:27460424 Regulation of RAW 264.7 cell-mediated immunity by polysaccharides from Agaricus blazei Murill via the MAPK signal transduction pathway PMCID: Pubmed No.:28322382 Pretreatment with low dose etomidate prevents etomidate-induced rat adrenal insufficiency by regulating oxidative stress-related MAPKs and apoptosis PMCID: Pubmed No.:25989534 Type II cyclic guanosine monophosphate-dependent protein kinase inhibits Rac1 activation in gastric cancer cells PMCID: Pubmed No.:26171059 Effects of Chronic REM Sleep Restriction on D1 Receptor and Related Signal Pathways in Rat Prefrontal Cortex PMCID: Pubmed No.:25793215 The regulation of SKF38393 on the signaling pathway of dopamine D1 receptor in hippocampus during chronic sleep deprivation PMCID: Pubmed No.:28583581 Combined treatment with vitamin E and gefitinib has synergistic effects to inhibit TGF-β1-induced renal fibroblast proliferation PMCID: Pubmed No.:27109695 Nitric oxide/cyclic guanosine monophosphate inducers sodium nitroprusside and L-arginine inhibit the proliferation of gastric cancer cells via the activation of type II cyclic guanosine monophosphate-dependent protein kinase PMCID: Pubmed No.:26171055 Signal transduction involved in lipoxin A4‑induced protection of tubular epithelial cells against hypoxia/reoxygenation injury PMCID: Pubmed No.:28259922 High PHLPP1 expression levels predicts longer time of acquired resistance to EGFR tyrosine kinase inhibitors in patients with lung adenocarcinoma PMCID: Pubmed No.:28938613 Establishment of a novel mesenchymal stem cell-based regimen for chronic myeloid leukemia differentiation therapy PMCID: Pubmed No.:33627636 Clemastine improves hypomyelination in rats with hypoxic–ischemic brain injury by reducing microglia-derived IL-1β via P38 signaling pathway PMCID: Pubmed No.:32061255 Brain‐derived neurotrophic factor: A steroidogenic regulator of Leydig cells PMCID: Pubmed No.:30628054 A phycocyanin derived eicosapeptide attenuates lung fibrosis development PMCID: Pubmed No.:34280398 Anti-Cancer Effects of Sulfasalazine and Vitamin E Succinate in MDA-MB 231 Triple-Negative Breast Cancer Cells PMCID: Pubmed No.:31171899 Effects of PHA-665752 and vemurafenib combination treatment on in vitro and murine xenograft growth of human colorectal cancer cells with BRAFV600E mutations PMCID: Pubmed No.:29456739 Ginsenoside Rg1 treatment alleviates renal fibrosis by inhibiting the NOX4–MAPK pathway in T2DM mice PMCID: Pubmed No.:37017270 IFN-γ enhances the therapeutic efficacy of MSCs-derived exosome via miR-126-3p in diabetic wound healing by targeting SPRED1 PMCID: Pubmed No.:37646268
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.