DOT1L, Chemiluminescent, BioAssay™ Kit (KMT4, KIAA1814, Histone Methyltransferase DOT1L)

The DOT1L Chemiluminescent BioAssay™ Kit is designed to measure DOT1L activity for screening and profiling applications.

Test Principle:
The key to the DOT1L Chemiluminescent BioAssay™ Kit is a highly specific antibody that recognizes methylated K79 residue of H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for two hours. Next, primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary antibody followed by addition of HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Applications:
Great for studying enzyme kinetics and HTS applications.

Kit Components:
Strip plate, 1x96 wells
DOT1L, 1x50ug
100uM S-adenosylmethionine, 1x250ul
DOT1L substrate, 1x250ul
Primary Antibody 25, 1x12.5ul
Secondary HRP-labeled Antibody 2, 1x10ul
4x HMT Assay Buffer 3, 1x3ml
Blocking Buffer, 1x50ml
HRP chemiluminescent substrate A, (translucent bottle), 1x6ml
HRP chemiluminescent substrate B, (brown bottle), 1x6ml

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.