DLL3 (Delta-like Protein 3, Drosophila Delta Homolog 3, Delta3)

DLL3, also known as Delta like ligand 3, is a transmembrane Delta-like protein principally found in the Golgi apparatus and endosomes where it promotes Notch family receptor degradation to inhibit primary neurogenesis. DLL3 is one of five DSL proteins that bind to the Notch receptor. Notch signaling pathways play key roles in cell-fate determination and differentiation in many tissues during embryonic and postnatal development. Notch ligands family members are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in the DLL3 gene causes autosomal recessive spondylocostal dysostosis.

Applications:
Suitable for use in Western Blot and Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.5ug/ml detected Delta3 on 10ug of human liver tissue lysate.
Flow Cytometry: 0.1ug detected Delta3 in HEK293 cells.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.