DEPC [1609-47-8]

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Cat# NB-64-35112-5g

Size : 5g

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Brand : Neo Biotech

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Phone : +1 850 650 7790

DEPC (Synonyms: Diethyl pyrocarbonate)

Catalog No. T65118 Copy Product Info

Purity: 99.30%

DEPC (Diethyl pyrocarbonate) is a potent and irreversible RNase inhibitor containing histidine residues.DEPC can be used to prevent RNA enzyme degradation by modifying histidine residues with carboxylation resulting in enzyme inactivation. DEPC showed inhibitory effect on the central chemical sensitivity of rabbits. DEPC modified His, Tyr, Ser and Thr residues.

DEPC

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Synonyms Diethyl pyrocarbonate

Cas No. 1609-47-8

Batch Information

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Purity:99.30%

Appearance:Liquid

Color:Transparent

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	DEPC (Diethyl pyrocarbonate) is a potent and irreversible RNase inhibitor containing histidine residues.DEPC can be used to prevent RNA enzyme degradation by modifying histidine residues with carboxylation resulting in enzyme inactivation. DEPC showed inhibitory effect on the central chemical sensitivity of rabbits. DEPC modified His, Tyr, Ser and Thr residues.
In vivo	Insulin binding to rat liver plasma membranes is inhibited in a time- and dose-dependent fashion by prior treatment of membranes with the histidine-specific reagent diethyl pyrocarbonate.[1]
Synonyms	Diethyl pyrocarbonate

Chemical Properties

Molecular Weight	162.14
Formula	C₆H₁₀O₅
Cas No.	1609-47-8
Smiles	CCOC(=O)OC(=O)OCC

Storage & Solubility Information

Storage

Pure form: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 55 mg/mL (339.21 mM), Sonication is recommended.

H2O: 30 mg/mL (185.03 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 5 mg/mL (30.84 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	6.1675 mL	30.8375 mL	61.6751 mL	308.3755 mL
5 mM	1.2335 mL	6.1675 mL	12.3350 mL	61.6751 mL
10 mM	0.6168 mL	3.0838 mL	6.1675 mL	30.8375 mL
20 mM	0.3084 mL	1.5419 mL	3.0838 mL	15.4188 mL
50 mM	0.1234 mL	0.6168 mL	1.2335 mL	6.1675 mL
100 mM	0.0617 mL	0.3084 mL	0.6168 mL	3.0838 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.