DBH ELISA Kit (Human)
Cat# OKCD07393
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
DBH ELISA Kit (Human) (OKCD07393)
| Datasheets/Manuals | Printable datasheet for DBH ELISA Kit (Human) (OKCD07393) |
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| Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
| ELISA Kit Duration | 3h | ||||||||||||||||||||||
| ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dopamine Beta Hydroxylase (DbH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dopamine Beta Hydroxylase (DbH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dopamine Beta Hydroxylase (DbH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dopamine Beta Hydroxylase (DbH) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
| ELISA Kit Range | 3.12-200ng/mL | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dopamine Beta Hydroxylase (DbH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dopamine Beta Hydroxylase (DbH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
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| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids | ||||||||||||||||||||||
| Sensitivity | < 1.31ng/mL |
| Gene Symbol | DBH |
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| Gene Full Name | dopamine beta-hydroxylase |
| Alias Symbols | DBM;dopamine beta-hydroxylase;dopamine beta-hydroxylase (dopamine beta-monooxygenase);Dopamine beta-monooxygenase;ORTHYP1. |
| NCBI Gene Id | 1621 |
| Protein Name | Dopamine beta-hydroxylase |
| Description of Target | Conversion of dopamine to noradrenaline. |
| Uniprot ID | P09172 |
| Protein Accession # | NP_000778.3 |
| Nucleotide Accession # | NM_000787.3 |
