D-myo-Inositol 1,4,5-trisphosphate (sodium salt) [108340-81-4]

D-myo-inositol 1,4,5-trisphosphate sodium salt is a widely distributed intracellular second messenger. D-myo-inositol 1,4,5-trisphosphate sodium salt acts as a potent agonist of the Ins (1,4,5) P₃ receptor with an EC₅₀ of 0.12 μM and inhibits bovine PLC-δ1, with an IC₅₀ of 5.4 μM for binding and an IC₅₀ of 12.4 μM for hydrolysis. D-myo-inositol 1,4,5-trisphosphate sodium saltis metabolized into Ins (1,4) P₂ and Ins (1,3,4,5) P₄. D-myo-inositol 1,4,5-trisphosphate sodium salt mediates various cellular processes, including growth, development, fertilization, secretion, contraction and neuromodulation. D-myo-inositol 1,4,5-trisphosphate sodium salt inhibits PIP₂ hydrolysis and Ca²⁺-ATPase activity. D-myo-inositol 1,4,5-trisphosphate sodium salt is used for cardiac preconditioning, exerts cardioprotective effects and mimics ischemic preconditioning. D-myo-inositol 1,4,5-trisphosphate sodium salt is applied in studies related to intracellular calcium and phosphoinositol signaling pathways^[1][2][3][4].

D-myo-inositol 1,4,5-trisphosphate sodium salt acts as the natural substrate for human erythrocyte membrane Ins(1,4,5)P3 5-phosphatase with a K_m of 40 μM, and potently mobilizes intracellular Ca²⁺ in electrically permeabilized human neuroblastoma cells with an EC₅₀ of 0.12 μM^[1].
D-myo-inositol 1,4,5-trisphosphate sodium salt acts as the natural substrate for crude rat brain Ins(1,4,5)P₃ 3-kinase with a K_m of 0.6 μM^[1].
D-myo-inositol 1,4,5-trisphosphate (15-60 μM; room temperature) sodium salt competitively inhibits the binding of PLC-δ₁ to PC/PIP₂ (98:2) LUVs with an IC₅₀ of 5.4 μM^[2].
D-myo-Inositol 1,4,5-trisphosphate (60 μM; 2-5 min preincubation for some experiments) sodium salt inhibits PLC-δ₁-catalyzed PIP₂ hydrolysis in PC/PIP₂ (95:5) LUVs with an IC₅₀ of 12.4 μM^[2].
D-myo-Inositol 1,4,5-trisphosphate (2 mM MgCl₂; 2 min) sodium salt is broken down by a Mg²⁺-dependent phosphatase in partially purified rat hepatocyte plasma membranes^[3].
D-myo-Inositol 1,4,5-trisphosphate (1.5 mM free Mg²⁺; 10 min at 37 °C) sodium salt phosphatase activity is almost exclusively localized to the plasma membrane fraction of rat liver^[3].
D-myo-Inositol 1,4,5-trisphosphate (1.0-5.0 mM calcium, manganese, putrescine, spermidine, spermine; 0.5-1.5 mM free Mg²⁺) sodium salt phosphatase activity in purified rat hepatocyte plasma membranes is Mg²⁺-dependent, with maximal activity at 0.5-1.5 mM free Mg^2+[3].
D-myo-Inositol 1,4,5-trisphosphate (1.5 mM free Mg²⁺; 1-60 min at 37 °C) sodium salt is degraded linearly for the first 20 min by purified rat hepatocyte plasma membranes, with approximately 65% of the substrate broken down after 60 min^[3].
D-myo-Inositol 1,4,5-trisphosphate sodium salt potently induces dose-dependent Ca²⁺ release from rat brain microsomes with an EC₅₀ of 0.13 μM, fully mobilizing the Ins(1,4,5)P₃-sensitive Ca²⁺ store^[4].
D-myo-Inositol 1,4,5-trisphosphate sodium salt potently displaces specific [³H]Ins(1,4,5)P₃ binding to rat cerebellar microsomes with an IC₅₀ of 0.031 μM, indicating strong affinity for the Ins(1,4,5)P₃ receptor^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

• [1]. Lampe D, et al. Synthesis of selective non-Ca(2+)-mobilizing inhibitors of D-myo-inositol 1,4,5-trisphosphate 5-phosphatase. J Med Chem. 1994;37(7):907-912.

  [2]. Cifuentes ME, et al. D-myo-inositol 1,4,5-trisphosphate inhibits binding of phospholipase C-delta 1 to bilayer membranes. J Biol Chem. 1994;269(3):1945-1948.  [Content Brief]

  [3]. Seyfred MA, et al. Characterization of D-myo-inositol 1,4,5-trisphosphate phosphatase in rat liver plasma membranes. J Biol Chem. 1984;259(21):13204-13208.

  [4]. Lu PJ, et al. Molecular interactions of endogenous D-myo-inositol phosphates with the intracellular D-myo-inositol 1,4,5-trisphosphate recognition site. Biochemistry. 1994;33(38):11586-11597.