CytoSelect 24-well Cell Migration and Invasion Assay (8 um), Fluorometric, Combo Kit

Principle of the assay: The CytoSelect Cell Migration Assay Kit contains polycarbonate membrane inserts (8 mum pore size) in a 24-well plate. The membrane serves as a barrier to discriminate migratory cells from non-migratory cells. Migratory cells are able to extend protrusions towards chemoattractants (via actin cytoskeleton reorganization) and ultimately pass through the pores of the polycarbonate membrane. These migratory cells are then dissociated from the membrane and subsequently detected by the patented CyQuant GR Dye (Invitrogen).

Background: Cell migration is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. It plays a pivotal role in the disease progression of cancer, mental retardation, atherosclerosis, and arthritis. The initial response of a cell to a migration-promoting agent is to polarize and extend protrusions in the direction of the attractant; these protrusions can consist of large, broad lamellipodia or spike-like filopodia. In either case, these protrusions are driven by actin polymerization and can be stabilized by extracellular matrix (ECM) adhesion or cell-cell interactions (via transmembrane receptors). The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the significant morbidity and mortality of cancers. Invasiveness requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Metastatic cells produce many proteolytic enzymes (e.g. lysosomal hydrolysates, collagenases, plasminogen activators) while the expression of certain cell surface protease receptors is also increased. CytoSelect Cell Migration and Invasion Assay utilize polycarbonate membrane inserts (8 mum pore size) or basement membrane-coated inserts to assay the migratory or invasive properties of cells. The kit does not require you to prelabel the cells with Calcein AM or remove non-migratory or non-invasive cells (i.e. cotton swabbing). Any migratory or invasive cells are first dissociated from the membrane, then lysed and detected by the patented CyQuant GR Dye (Invitrogen). TheCytoSelect Cell Migration and Invasive Assay Kit provides a robust system for the quantitative determination of cell migration. Each assay contains sufficient reagents for the evaluation of 12 samples. The 8 mum pore size is optimal for epithelial and fibroblast cell migration. However, in the case of leukocyte chemotaxis, a smaller pore size (3 mum) is recommended.

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Small volumes of CytoSelect Cell Migration and Invasion Assay (8 um) assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS169058 is an assay kit targeting CytoSelect Cell Migration and Invasion Assay (8 um). Also known as CytoSelect 24-well Cell Migration and Invasion Assay (8 um), Fluorometric, Combo Kit.