Clec7a rat monoclonal antibody, clone 2A11, Low Endotoxin
Size:
500 ug
Conjugation:
Unconjugated
View other "Clec7a or NP_064392.2" antibodies
Specifications
Product Data
Clone Name
2A11
Application
FC, FN, IHC, IP
Recommended Dilution
Functional Assays. Immunoprecipitation. Flow Cytometry: Use 1/10-1/50 diluted antibody to label 106 cells in 100 µl. Immunohistochemistry on Frozen Sections: The epitope recognised by 2A11 is sensitive to H202 treatment, therefore quenching with 0.3% H202 should be performed after incubation with the primary antibody.
Reactivity
Mouse
Antibody Host
Rat
Isotype
IgG2b
Clonality
Monoclonal
Immunogen
Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1. Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line.
Specificity
This antibody clone 2A11 recognises beta-Glucan Receptor, also known as Dectin-1. This antibody inhibits the binding of zymosan to macrophages via the beta-glucan receptor. The low endotoxin version (SM2025LE) is recommended for functional studies.
Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells. As a major leucocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens. Recent work has shown that Dectin-1 may stimulate reactive oxygen production in macropahges via the protein tyrosine kinase known as Syk.
Synonyms
Beta-glucan receptor, BGR, CLECSF12, DECTIN1
Note
Endotoxin Level: Less than 0.01 EU/µg
Reference Data
Protein Categories
CD Markers, Cytokines, Immune system diseases, Intracellular Proteins, Membrane Proteins
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
