Chicken secretory immunoglobulin A,SIgA ELISA Kit
Cat# CSB-E10097Ch-96T
Size : 96T
Brand : Cusabio
Contact local distributor :
Phone : +1 850 650 7790
| Size | 96T,5×96T,10×96T |
Product Details
Target Name
secretory immunoglobulin A,SIgA
Abbreviation
SIgA
Species
Gallus gallus (Chicken)
Sample Types
serum, plasma
Detection Range
0.234 μg/mL-60 μg/mL
Sensitivity
0.058 μg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Competitive
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken SIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 86 | ||||
| Range % | 82-89 | |||||
| 1:400 | Average % | 98 | ||||
| Range % | 92-104 | |||||
| 1:800 | Average % | 97 | ||||
| Range % | 93-101 | |||||
| 1:1600 | Average % | 88 | ||||
| Range % | 84-92 | |||||
Recovery
| The recovery of chicken SIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-95 | ||||
| EDTA plasma (n=4) | 104 | 98-108 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Chicken SIgA ELISA Kit was designed for the quantitative measurement of Chicken SIgA protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 0.234 μg/mL-60 μg/mL and the sensitivity is 0.058 μg/mL.
Citations
- C Yu,Italian Journal of Animal Science,2022
- JF Chen,Poultry Science,2020
- Chen JF,Journal of Animal Physiology and Animal Nutrition,2019
- Qiujuan Sun. et al,Animal Feed Science and Technology,2015
Customer Reviews and Q&A
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■ Q&A
Q:
For the kit CSB-E10097Ch,may I know if it can be tested with tissue samples, specifically small intestine?
Would you have this information available to share?
A:
Thanks for your inquiry.
We suggest you do a pretest if you want to test small intestine.Pls let me know if you have any further questions. Thank you.
We suggest you do a pretest if you want to test small intestine.Pls let me know if you have any further questions. Thank you.