Images
Immunocytochemistry
Application

Immunocytochemistry

Immunocytochemistry analysis of CDK9(green) in Hela using human CDK9 recombinant monoclonal antibody, clone R03-5B9 (Cat # RAB01511), and DAPI(blue).

Western Blot
Application

Western Blot

Western blot analysis of CDK9 in K562, C6, 3T3, Hela lysates using human CDK9 recombinant monoclonal antibody, clone R03-5B9 (Cat # RAB01511).

  • Specifications

    Product Description

    Rabbit recombinant monoclonal antibody raised against synthetic peptide of human Cdk9.Recombinant Antibody,Recombinant Antibodies,Recombinant Monoclonal Antibody,RecomAb,Recombinant Ab,Recombinant Monoclonal Antibodies,Recombinant Abs,RAB,MAB,Recombinant Mab,MAB,monoclonal Ab,monoclonal antibody

    Antibody Species

    Rabbit

    Immunogen

    Original antibody is raised against a synthetic peptide corresponding to human Cdk9

    Theoretical MW (kDa)

    Calculated MW: 43 kD

    Reactivity

    Human

    Form

    Liquid

    Preparation Method

    Mammalian cell (CHO) expression system

    Purification

    Affinity purification

    Isotype

    IgG

    Recommend Usage

    Immunofluorescence (1:50-1:200)
    Immunohistochemistry (1:50-1/100)
    Immunoprecipitation (1:20)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In 50 mM Tris-Glycine pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA).

    Storage Instruction

    Store at 4°C for short term. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemistry (Frozen sections)

    Immunocytochemistry

    Immunocytochemistry analysis of CDK9(green) in Hela using human CDK9 recombinant monoclonal antibody, clone R03-5B9 (Cat # RAB01511), and DAPI(blue).

    Immunoprecipitation

    Western Blot

    Western blot analysis of CDK9 in K562, C6, 3T3, Hela lysates using human CDK9 recombinant monoclonal antibody, clone R03-5B9 (Cat # RAB01511).
  • Gene Info — CDK9

    Entrez GeneID

    1025

    Protein Accession#

    P50750

    Gene Name

    CDK9

    Gene Alias

    C-2k, CDC2L4, CTK1, PITALRE, TAK

    Gene Description

    cyclin-dependent kinase 9

    Omim ID

    603251

    Gene Ontology

    Hyperlink

    Gene Summary

    The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq

    Other Designations

    CDC2-related kinase|OTTHUMP00000022198|cell division protein kinase 9|serine/threonine protein kinase PITALRE

  • Interactomes
eProcurement