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> CD73/5'-Nucleotidase Protein, Mouse, Recombinant (His)

CD73/5'-Nucleotidase Protein, Mouse, Recombinant (His)

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Cat# NB-64-56492-5ug

Size : 5ug

Brand : Neo Biotech

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CD73/5'-Nucleotidase Protein, Mouse, Recombinant (His)

(Synonyms: Nt5, NT, eNT, CD73, AI447961, 5'-nucleotidase, ecto (CD73), 2210401F01Rik) Copy Product Info

Synonyms: Nt5, NT, eNT, CD73, AI447961, 5'-nucleotidase, ecto (CD73), 2210401F01Rik

Catalog No. TMPY-01342 Copy Product Info
CD73 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.4 kDa and the accession number is Q0VEE0.
Other Forms of CD73/5'-Nucleotidase Protein, Mouse, Recombinant (His):
NTS1 Protein, Mouse, Recombinant (hFc)
Neurotensin Protein, Human, Recombinant (hFc)
CD73/5'-Nucleotidase Protein, Human, Recombinant (His)
CD73/5'-Nucleotidase Protein, Cynomolgus, Recombinant (His)
CD73/5'-Nucleotidase Protein, Rat, Recombinant (His)
CD73/5'-Nucleotidase Protein, Human, Recombinant (hFc)
NT-4 Protein, Human, Recombinant
CD73 Protein, Human, Recombinant (His & Avi), Biotinylated
For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:92.9%
Appearance:Lyophilized powder
Color:White
Contact us for more batch information

Product Introduction

Bioactivity
Bioactivity
Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit . The specific activity is >10,000 pmol/min/µg.
Description
CD73 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.4 kDa and the accession number is Q0VEE0.
Species
Mouse
Expression System
HEK293 Cells
TagC-His
Accession NumberQ61503
ConstructionTrp29-Lys549
Protein Purity
> 95 % as determined by SDS-PAGE
CD73/5'-Nucleotidase Protein, Mouse, Recombinant (His)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation1. Lyophilized from 20 mM Tris, 500 mM NaCl, pH 7.4. <br> 2. Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 120 mM NaCl (pH 7.5). <br> Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsNt5, NT, eNT, CD73, AI447961, 5'-nucleotidase, ecto (CD73), 2210401F01Rik
Research Background
5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5'-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signaling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purinergic receptors to induce cellular responses. CD73 has also been reported to regulate the expression of pro-inflammatory molecules in mouse endothelium.
Chemical Properties
Molecular Weight59.4 kDa (predicted); 59.4 kDa (reducing conditions)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

CD-73CD 73AI-447961AI 4479615'-nucleotidase, ecto
Related Tags: CD73/5'-Nucleotidase Protein, Mouse, Recombinant (His) chemical structure | CD73/5'-Nucleotidase Protein, Mouse, Recombinant (His) molecular weight