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> CD45R (B220, Ly-5, Lyt-4) (APC/Cy7)

CD45R (B220, Ly-5, Lyt-4) (APC/Cy7)

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Cat# 228126-100ug

Size : 100ug

Brand : US Biological

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Phone : +1 850 650 7790

228126 CD45R (B220, Ly-5, Lyt-4) (APC/Cy7)

Clone Type
Monoclonal
Host
rat
Source
mouse
Isotype
IgG2a
Grade
Purified
Applications
FC IHC
Crossreactivity
Fe Hu Mo
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

CD45R, also known as B220, a member of the protein tyrosine phosphate family and a major cell surface glycoprotein, represents a restricted form of the CD45 family which primarily recognizes only cells of B-lineage from pro-B cell through mature B lymphocytes and prior to the availability of anti-CD19 monoclonal antibodies was commonly used as a pan B-cell marker. It also reacts with certain activated T cells as well as non-MHC-restricted lytically active lymphokine-activated killer (LAK) cells. In vivo administration has been shown to affect differentiation of both T and B cells in normal mice and reduce the level of anti-DNA antibodies and lymphadenopathies in MRL/lpr mice.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 0.2ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC/Cy7 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG2a|Clone No: RA3-6B2|Host: rat|Source: mouse|Concentration: ~0.1mg/ml |Form: Supplied as a liquid in PBS, sodium azide. Labeled with Allophycocyanin (APC/Cy7).|Purity: Purified|Immunogen: Abelson murine leukemia virus-induced pre-B cell lymphoma.|Specificity: Recognizes mouse CD45R. Species Crossreactivity: human, feline||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Abelson murine leukemia virus-induced pre-B cell lymphoma.
Form
Supplied as a liquid in PBS, sodium azide. Labeled with Allophycocyanin (APC/Cy7).
Purity
Purified
Specificity
Recognizes mouse CD45R. Species Crossreactivity: human, feline
References
1. Coffman RL. Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development. Immunol Rev. 1982;69:5- 23. (Immunogen, FC, IP, Block, Human Reactivity) 2. Cappione AJ, Pugh-Bernard AE, Anolik JH, Sanz I. Lupus IgG VH4.34 antibodies bind to a 220-kDa glycoform of CD45/B220 on the surface of human B lymphocytes. J Immunol. 2004;172:4298-307. (IP, WB, Human Reactivity)3.Monteith CE, Chelack BJ, Davis WC, Haines DM. Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues. Can J Vet Res. 1996;60:193-8. (IHC-FS, IHC-PS, FC, Feline Reactivity) 4. Fütterer A, Campanero MR, Leonardo E, Criado LM, Flores JM, Hernández JM, et al. Dido gene expression alterations are implicated in the induction of hematological myeloid neoplasms. J Clin Invest.2005;115:2351-62. (IHC-PS) 5.Konno A, Takada K, Saegusa J, Takiguchi M. Presence of B7-2+ dendritic cells and expression of Th1 cytokines in the early development of sialodacryoadenitis in the IQI/Jic mouse model of primary Sjörgren's syndrome. Autoimmunity. 2003;36:247-54. (IHC-FS)|6.Haub S, Ritze Y, Bergheim I, Pabst O, Gershon MD, Bischoff SC. Enhancement of intestinal inflammation in mice lacking interleukin 10 by deletion of the serotonin reuptake transporter. Neurogastroenterol Motil. 2010;22:826-34. (IHC-FS) 7. Nepal RM, Tong L, Kolaj B, Edelmann W, Martin A. Msh2-dependent DNA repair mitigates a unique susceptibility of B cell progenitors to c-Myc- induced lymphomas. Proc Natl Acad Sci USA. 2009;106:18698-703. (FC) 8. Birjandi SZ, Ippolito JA, Ramadorai AK, Witte PL. Alterations in marginal zone macrophages and marginal zone B cells in old mice. J Immunol. 2011;186:3441-51. (FC) 9. King RG, Herrin BR, Justement LB. Differential expression of the adaptor protein HSH2 controls the quantitative and qualitative nature of the humoral response. J Immunol. 2011;187:3565-77. (FC) 10. Hinze A, Stolzing A. Microglia differentiation using a culture system for the expansion of mice non-adherent bone marrow stem cells. J Inflamm. 2012;9:12. (FC) 11. Berge T, Grønningsæter IH, Lorvik KB, Abrahamsen G, Granum S, Sundvold-Gjerstad V, et al. SH2D2A modulates T cell mediated protection to a B cell derived tumor in transgenic mice. PLoS One. 2012;7(10):e48239. (FC) 12. Alkhatib A, Werner M, Hug E, Herzog S, Eschbach C, Faraidun H, et al. FoxO1 induces Ikaros splicing to promote immunoglobulin gene recombination. J Exp Med. 2012;209:395-406. (FC) 13. Grodeland G, Mjaaland S, Roux KH, Fredriksen AB, Bogen B. DNA vaccine that targets hemagglutinin to MHC class II molecules rapidly induces antibody-mediated protection against influenza. J Immunol. 2013;191:3221-31. (FC) 14. Jacobsen J, Haabeth OW, Tveita AA, Schjetne KW, Munthe LA, Bogen B. Naive idiotope-specific B and T cells collaborate efficiently in the absence of dendritic cells. J Immunol. 2014;192:4174-83. (FC)