Immunofluorescence
Immunofluorescent staining of Daudi cells with CD40 recombinant monoclonal antibody, clone 5D12 (Cat # RAB03266).
Daudi cells on Shi-fix™ coverslips- permewith 0.15% Triton and stained with the chimeric r version of RAB03266 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor® 488 secondary antibody.
- Specifications
Product Description
Rabbit recombinant monoclonal antibody raised against human CD40.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against sf9 insect cells expressing recombinant human CD40.
Reactivity
Crab-eating macaque, Human, Marmoset
Form
Liquid
Preparation Method
Mammalian cell (HEK293) expression system
Isotype
IgG kappa
Recommend Usage
Blocking
ELISA
Flow Cytometry
Immunofluorescence
Immunohistochemistry
Immunoprecipitation
Surface Plasmon Resonance
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% Proclin 300)
Storage Instruction
Store at 4°C for 3 months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. - Applications
Immunohistochemistry
Immunofluorescence
Immunofluorescent staining of Daudi cells with CD40 recombinant monoclonal antibody, clone 5D12 (Cat # RAB03266).
Daudi cells on Shi-fix™ coverslips- permewith 0.15% Triton and stained with the chimeric r version of RAB03266 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor® 488 secondary antibody.Flow Cytometry
Enzyme-linked Immunoabsorbent Assay
Immunoprecipitation
Blocking
Surface Plasmon Resonance
- Gene Info — CD40
Entrez GeneID
958Gene Name
CD40
Gene Alias
Bp50, CDW40, MGC9013, TNFRSF5, p50
Gene Description
CD40 molecule, TNF receptor superfamily member 5
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
B cell surface antigen CD40|B cell-associated molecule|CD40 antigen|CD40 antigen (TNF receptor superfamily member 5)|CD40 type II isoform|CD40L receptor|OTTHUMP00000031699|OTTHUMP00000031700|nerve growth factor receptor-related B-lymphocyte activation mol
- Interactomes
- Pathways
- Diseases