CD11b (Mac-1, Mo1, CR3, C3biR) (APC/Cy7)

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Cat# 228046-100ug

Size : 100ug

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Phone : +1 850 650 7790

Brand : US Biological

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Contact local distributor :

Phone : +1 850 650 7790

228046 CD11b (Mac-1, Mo1, CR3, C3biR) (APC/Cy7)

Clone Type

Monoclonal

Host

rat

Source

mouse

Swiss Prot

G5E8F1

Isotype

IgG2b

Grade

Purified

Applications

Crossreactivity

Hu Mk Mo Rb

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

CD11b, the integrin M subunit, combines with CD18, the integrin 2 subunit, to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1/CD54. Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities. Mac-1 expression is rapidly up-regulated on neutrophils after activation and in the same time period that CD62L is shed from the cell surface. In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process, Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils. Cross-reaction to human CD11b has been observed on monocytes, polymorphonuclear leukocytes, and NK cells.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 0.3ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC/Cy7 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Applications

Product Type: Mab|Isotype: IgG2b|Clone No: M1/70|Host: rat|Source: mouse|Concentration: ~0.1mg/ml |Form: Supplied as a liquid in PBS, sodium azide. Labeled with Allophycocyanin (APC/Cy7).|Purity: Purified|Immunogen: C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes.|Specificity: Recognizes mouse CD11. Species Crossreactivity: Human, Rhesus, Baboon, Rabbit ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes.

Form

Supplied as a liquid in PBS, sodium azide. Labeled with Allophycocyanin (APC/Cy7).

Purity

Purified

Specificity

Recognizes mouse CD11. Species Crossreactivity: Human, Rhesus, Baboon, Rabbit

References

1. Springer T, Galfré G, Secher DS, Milstein C. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur J Immunol. 1978;8:539-51. (Immunogen, IP) 2.Springer T, Galfré G, Secher DS, Milstein C. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur J Immunol. 1979;9:301-6. (IP, FC) 3.Igarashi T, Miyake K, Hayakawa J, Kawabata K, Ishizaki M, Takahashi H, et al. Apoptotic cell death and regeneration in the newborn retina after irradiation prior to bone marrow transplantation. Current Eye Res. 2007;32:543-53. (IHC-FS) 4.Kosuge H, Ishihara T, Haraguchi G, Maejima Y, Okada H, Saiki H, et al. Treatment with telmisartan attenuates graft arteriosclerosis in murine cardiac allografts. J Heart Lung Transplant. 2010;29:562-7. (IHC-FS) 5.Ault KA, Springer TA. Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells. J Immunol. 1981;126:359-64. (FC, Human Reactivity) 6.Beller DI, Springer TA, Schreiber RD. Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor. J Exp Med. 1982;156:1000-9. (Block, Human Reactivity) 7.Martin A, Le Corre R, Pellen P, Bourel D, Merdrignac G, Genetet B, et al. A monoclonal antibody (3A33) that reacts with a mouse-specific epitope of Mac-1 antigen. Tissue Antigens. 1986;28:15-23. (IP, FC) 8.Pevsner-Fischer M, Morad V, Cohen-Sfady M, Rousso-Noori L, Zanin-Zhorov A, Cohen S, et al. Toll-like receptors and their ligands control mesenchymal stem cell functions. Blood. 2007;109:1422-32. (FC) 9.Barton RW, Rothlein R, Ksiazek J, Kennedy C. The effect of anti-intercellular adhesion molecule-1 on phorbol-ester-induced rabbit lung inflammation. J Immunol. 1989;143:1278-82. (FC, Rabbit Reactivity) 10.Marcondes MC, Flynn C, Huitron-Rezendiz S, Watry DD, Zandonatti M, Fox HS. Early antiretroviral treatment prevents the development of central nervous system abnormalities in simian immunodeficiency virus-infected rhesus monkeys. AIDS. 2009;23:1187-95. (FC, Rhesus Reactivity) 11. del Zappo GJ, Frankowski H, Gu Y, Osada T, Kanazawa M, Milner R, et al. Microglial cell activation is a source of metalloproteinase generation during hemorrhagic transformation. J Cereb Blood Flow Metab. 2012;32:919-32. (Baboon Reactivity)