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> CaM Kinase II, alpha (Calmodulin-Dependent Protein Kinase II) (PE)

CaM Kinase II, alpha (Calmodulin-Dependent Protein Kinase II) (PE)

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Cat# C1036-78-PE-100ul

Size : 100ul

Brand : US Biological

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Phone : +1 850 650 7790

C1036-78-PE CaM Kinase II, alpha (Calmodulin-Dependent Protein Kinase II) (PE)

Clone Type
Monoclonal
Host
mouse
Source
rat
Isotype
IgG2a,k
Grade
Affinity Purified
Applications
FLISA IHC IP WB
Crossreactivity
Hu Mo Rt Sh
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

CaM Kinase II (Ca 2+ /calmodulin-dependent protein kinase II) is a multifunctional Ca 2+ /calmodulin-dependent serine/threonine kinase which is able to phosphorylate a variety of proteins in response to Ca 2+ signals. This kinase participates in the regulation of numerous cellular functions including: 1) metabolism of carbohydrates, lipids, and amino acids, 2) neurotransmitter synthesis and release, 3) regulation of ion flux through ion channels, 4) Ca 2+ homeostasis, 5) cytoskeletal function, and 6) Ca 2+ -regulated changes in gene expression. CaM kinase II is broadly expressed and is highly concentrated in brain particularly in cortical structure and hippocampus. This kinase also plays an essential role in induction of long-term potentiation. Purified rat brain CaM kinase has an apparent molecular weight of ~540kD and consists of isozymes composed of various ratios of 54kD (a), 58kD (b’), 60kD (b), 59kD (g) and 60kD (d) in a holoenzyme containing 10–12 subunits. The primary difference in the subunit isoforms lies in the variable region located at the carboxyl-end of the calmodulin binding domain. Ca 2+ /calmodulin-stimulated autophosphorylation of CaM kinase II occurs on multiple residues. In particular, autophosphorylation on residue Thr 286 /Thr 287 within the regulatory domain results in full activation of the enzyme, and in the generation of Ca 2+ /calmodulin-independent activity. Autophosphorylation has also been reported to increase the affinity of CaM kinase for Ca 2+ /calmodulin.

Applications:
Suitable for use in FLISA, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Paraffin sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. 

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG2a,k|Clone No: 3G55|Host: mouse|Source: rat|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with R-Phycoerythrin (PE).|Purity: Purified by immunoaffinity chromatography.|Immunogen: Purified rat brain CaM Kinase.|Specificity: Recognizes the alpha subunit of rat CaM Kinase II. No significant crossreactivity was seen with the Beta, Beta1, gammaB, epsilonA, epsilonB and epsilonC isoforms. The epitope is located in the regulatory domain. Species Crossreactivity: human, mouse, sheep and fish.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Purified rat brain CaM Kinase.
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with R-Phycoerythrin (PE).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes the alpha subunit of rat CaM Kinase II. No significant crossreactivity was seen with the Beta, Beta1, gammaB, epsilonA, epsilonB and epsilonC isoforms. The epitope is located in the regulatory domain. Species Crossreactivity: human, mouse, sheep and fish.
References
1. Hanson, P.I., Shulman, H.: Neuronal Ca 2+ /calmodulin-dependent protein kinases. Annu. Rev. Biochem. 61: 559–601 (1992). 2. Scholz, W.K., et al.: Developmental changes in Ca 2+/ calmodulin-dependent protein kinase II in cultures of hippocampal pyreamidal neurons and astrocytes. J. Neuroscience 8: 1039-1051 (1988). 3. Baitinger, C. et al.: Multifunctional Ca 2+/ calmodulin-dependent protein kinase II is necessary for nuclear envelope breakdown. J. Cell. Biology 111: 1763-1773 (1990). 4. Brocke, L., et al.: J. Neuroscience 15(10): 6797-6808 (1995). 5. Chang, B.H., et al.: PNAS I95: 10890-10895 (1998).